You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-PRMT5 antibody (ab3766)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-PRMT5 antibody
See all PRMT5 products (8) ...

Description

Rabbit polyclonal to PRMT5

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide: TNLARVLTNHIHTGHHSS , corresponding to amino acids 132-149 of Human PRMT 5.

TNLARVLTNHIHTGHHSS

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS with 0.1% sodium azide

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Arginine Methylation
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation

  • Western blot - PRMT5 antibody (ab3766)Western blot - PRMT5 antibody (ab3766) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the protein arginine N-methyltransferase family.

Post-translational
modifications

Disulfide bonds and non-covalent association mediate homooligomers formation.

Cellular localization

Cytoplasm. Nucleus.

Target information above from: UniProt accessionO14744 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • HOMOLOG OF; SKB1 antibody
  • 72 kDa ICln binding protein antibody
  • 72 kDa ICln-binding protein antibody
  • ANM5_HUMAN antibody
  • Histone-arginine N-methyltransferase PRMT5 antibody
  • HMT1 hnRNP methyltransferase like 5 antibody
  • HOMOLOG OF; SKB1 antibody
  • HRMT1L5 antibody
  • IBP72 antibody
  • Jak-binding protein 1 antibody
  • JBP 1 antibody
  • JBP1 antibody
  • PRMT 5 antibody
  • PRMT5 antibody
  • Protein arginine methyltransferase 5 antibody
  • Protein arginine N methyltransferase 5 antibody
  • Protein arginine N-methyltransferase 5 antibody
  • S. POMBE antibody
  • S. POMBE antibody
  • S. POMBE HOMOLOG OF; SKB1 antibody
  • SHK1 KINASE BINDING PROTEIN 1 antibody
  • Shk1 kinase binding protein 1 homolog antibody
  • SHK1 KINASE-BINDING PROTEIN 1 antibody
  • Shk1 kinase-binding protein 1 homolog antibody
  • SKB 1 antibody
  • SKB1 antibody
  • SKB1 homolog antibody
  • SKB1: SKB1 homolog (S. pombe) antibody
  • SKB1Hs antibody
see all

Database links

Anti-PRMT5 antibody images:

  Western blot - PRMT5 antibody (ab3766)

Western blot - PRMT5 antibody (ab3766)



Predicted band size : 72,684 kDa


Western blot analysis using whole HL60 cell lysate: PRMT5 (Arrow) was detected using ab3766 (Lane B) but not pre-immune serum (lane A). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

References for Anti-PRMT5 antibody (ab3766)

This product has been referenced in:

  • Dolzhanskaya N  et al. Alternative splicing modulates protein arginine methyltransferase-dependent methylation of fragile X syndrome mental retardation protein. Biochemistry 45:10385-93 (2006).Read more (PubMed: 16922515) »

See 1 publication for this product

Publishing research using ab3766? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"