You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameAnti-PRMT6 antibodySee all PRMT6 primary antibodies ...
  • Description
    Rabbit polyclonal to PRMT6
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human PRMT6.

    Read Abcam's proprietary immunogen policy

  • Positive controlThis antibody gave a positive signal in Human Testis as well as A498 and HeLa whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab104834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • FunctionArginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC).
  • Tissue specificityHighly expressed in kidney and testis.
  • Sequence similaritiesBelongs to the protein arginine N-methyltransferase family. PRMT6 subfamily.
  • Post-translational
    modifications    Automethylated.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q96LA8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      ANM6_HUMAN antibodyFLJ10559 antibodyFLJ51477 antibody
      Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6 antibodyHeterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6 antibodyHistone-arginine N-methyltransferase PRMT6 antibodyHMT1 hnRNP methyltransferase like 6. antibodyHRMT1L6 antibodyOTTHUMP00000012633 antibodyPRMT 6 antibodyPRMT 6 antibodyprmt6 antibodyProtein arginine methyltransferase 6 antibodyProtein arginine N methyltransferase 6 antibodyProtein arginine N-methyltransferase 6 antibody
    see all

Anti-PRMT6 antibody images

  • Western blot - Anti-PRMT6 antibody (ab104834)
    Western blot - Anti-PRMT6 antibody (ab104834)
    All lanes : Anti-PRMT6 antibody (ab104834) at 1 µg/ml

    Lane 1 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 120 kDa,68 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References for Anti-PRMT6 antibody (ab104834)

ab104834 has not yet been referenced specifically in any publications.

Publishing research using ab104834 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab104834 Submit a Question for ab104834

There are currently no Abreviews or Questions for ab104834.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"