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Anti-PRMT6 antibody (ab12192)

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Overview

Product name

Anti-PRMT6 antibody
See all PRMT6 products (10) ...

Description

Rabbit polyclonal to PRMT6

Specificity

Anti PRMT6 (SE-15) antibody reacts specifically with PRMT6.

Tested applications

WB, IF, IPmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide: SQPKKRKLESGGGGE conjugated to KLH via a C-terminal added cysteine, corresponding to amino acids 2-16 of Human PRMT6.

SQPKKRKLESGGGGE

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Immunoblotting/Immunoprecipitation: cell extracts of PRMT6 transfected 293T cells. Immunofluorescence: paraformaldehyde fixed HeLa cells.

General notes

If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody is affinity purified on the immunizing peptide immobilized on agarose.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Arginine Methylation
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing

Applications

Show applications key

Our Abpromise guarantee covers the use of ab12192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

  • IF

     

  • IP

     

Application notes

IF: Use at a concentration of 3 - 4 µg/ml (recommended using paraformaldehyde fixed HeLa cells).
IP: 2.5 - 5 µg of the antibody immunoprecipitates PRMT6 protein from extracts of PRMT6 transfected 293T cells.
WB: Use at a concentration of 0.25 - 0.5 µg/ml (recommended using cell extracts of PRMT6 transfected 293T cells). Staining of the PRMT6 band in immunoblotting is specifically inhibited by the PRMT6 immunizing peptide. Predicted molecular weight: 38 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC).

Tissue specificity

Highly expressed in kidney and testes.

Sequence similarities

Belongs to the protein arginine N-methyltransferase family. PRMT6 subfamily.

Post-translational
modifications

Automethylated.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ96LA8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • PRMT 6 antibody
  • ANM6_HUMAN antibody
  • FLJ10559 antibody
  • FLJ51477 antibody
  • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6 antibody
  • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6 antibody
  • Histone-arginine N-methyltransferase PRMT6 antibody
  • HMT1 hnRNP methyltransferase like 6. antibody
  • HRMT1L6 antibody
  • OTTHUMP00000012633 antibody
  • prmt6 antibody
  • Protein arginine methyltransferase 6 antibody
  • Protein arginine N methyltransferase 6 antibody
  • protein arginine N-methyltransferase 6 antibody
see all

Database links

References for Anti-PRMT6 antibody (ab12192)

This product has been referenced in:

  • Yildirim AO  et al. Increased protein arginine methylation in chronic hypoxia: role of protein arginine methyltransferases. Am J Respir Cell Mol Biol 35:436-43 (2006). WB; Mouse.Read more (PubMed: 16690984) »

See 1 publication for this product

Publishing research using ab12192? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"