Anti-PRMT7 antibody (ab126965)
Key features and details
- Rabbit polyclonal to PRMT7
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PRMT7 antibody
See all PRMT7 primary antibodies -
Description
Rabbit polyclonal to PRMT7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 408-665 of Human PRMT7 (Q9NVM4).
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Positive control
- PC3 whole cell lysate; Rat RT2, Hela and Mouse C2C12 xenograft tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 78 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 78 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo. -
Involvement in disease
Defects in PRMT7 are associated with mild intellectual disabilityy, obesity and symmetrical shortening of the digits and posterior metacarpals and metatarsals. The phenotype is a phenocopy of pseudohypoparathyroidism (PHP). -
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily.
Contains 2 SAM-dependent MTase PRMT-type domains. -
Cellular localization
Cytoplasm, cytosol. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 54496 Human
- Entrez Gene: 214572 Mouse
- Entrez Gene: 361402 Rat
- Omim: 610087 Human
- SwissProt: Q9NVM4 Human
- SwissProt: Q922X9 Mouse
- SwissProt: Q5U4E8 Rat
- Unigene: 640229 Human
see all -
Alternative names
- [Myelin basic protein]-arginine N-methyltransferase PRMT7 antibody
- ANM7_HUMAN antibody
- FLJ10640 antibody
see all
Images
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Anti-PRMT7 antibody (ab126965) at 1/2000 dilution + PC3 whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 78 kDa
7.5% SDS PAGE -
ab126965, at 1/500 dilution, staining PRMT7 in Paraffin-embedded Rat RT2 xenograft tissue by Immunohistochemistry.
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ab126965, at 1/500 dilution, staining PRMT7 in Paraffin-embedded Human Hela xenograft tissue by Immunohistochemistry.
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ab126965, at 1/500 dilution, staining PRMT7 in Paraffin-embedded Mouse C2C12 xenograft tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab126965 has been referenced in 1 publication.
- Gurung B et al. Menin epigenetically represses Hedgehog signaling in MEN1 tumor syndrome. Cancer Res 73:2650-8 (2013). WB . PubMed: 23580576