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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Non Homol. End Joining
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab27692? Please let us know so that we can cite the reference in this datasheet
ab27692 has been referenced in 2 publications.
- Sato M et al. Human PRP19 interacts with prolyl-hydroxylase PHD3 and inhibits cell death in hypoxia. Exp Cell Res 316:2871-82 (2010). WB, IP; Human. PubMed: 20599946
- Kleinridders A et al. PLRG1 is an essential regulator of cell proliferation and apoptosis during vertebrate development and tissue homeostasis. Mol Cell Biol 29:3173-85 (2009). WB; Mouse. PubMed: 19307306
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - PRP19 antibody (ab27692)
All lanes : Anti-PRP19 antibody (ab27692) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Lane 3 :
Lysates/proteins at 20 µg per lane.
Secondary
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 55 kDa
Observed band size : 55 kDa
Immunocytochemistry/ Immunofluorescence - PRP19 antibody (ab27692)
Ab27692, at 1/500 dilution, staining human PRP 19 in HeLa Cells by ICC/IF.
This image is part of an abreview submitted by Dr Kirk McManus
Western blot - PRP19 antibody (ab27692)
All lanes : Anti-PRP19 antibody (ab27692) at 1 µg/ml
Lane 1 :
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 55 kDa
Observed band size : 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRP19 antibody (ab27692)
Image courtesy of Human Protein Atlas. ab27692 staining PRP19 in human breast cancer, showing a distinct nuclear staining pattern. Paraffin embedded human breast cancer tissue was incubated with ab27692 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab27692 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
PRP19 antibody for ICC/IF in Human (27692)
Anti-PRP19 antibody for Immunocytochemistry/ Immunofluorescence in Xenopus laevis (27692)
Anti-PRP19 antibody for Western blot in Xenopus laevis (27692)
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