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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Non Homol. End Joining
Overview
Product name
Anti-PRP19 antibody
See all PRP19 products (4) ...
Description
Rabbit polyclonal to PRP19
Tested applications
IHC-P, WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human PRP19.
(Peptide available as ab28710.)
Positive control
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate A431 (Human epithelial carcinoma cell line) Whole Cell Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IP: Use at an assay dependent dilution. (PubMed: 20599946)
ICC/IF: 1/500
Target
Relevance
PRP19 is a homolog of the protein encoded by PRP19/PSO4 of Saccharomyces cerevisiae, and plays a major role in mammalian DNA DSB repair. PRP19 has pleiotropic functions in DNA recombination and error prone repair. It binds double stranded DNA nonspecifically, but does not bind single stranded DNA. It is induced 15 to 30 fold in cells by gamma radiation and chemical mutagens. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.
Cellular localization
Cytoplasmic and Nuclear, mitotic spindle during anaphase.
Alternative names
- hPSO4 antibody
- NMP200 antibody
- nuclear matrix protein 200 antibody
see all
Database links
- Entrez Gene: 27339 Human
- Entrez Gene: 28000 Mouse
- Entrez Gene: 246216 Rat
- Omim: 608330 Human
- SwissProt: Q9UMS4 Human
- SwissProt: Q99KP6 Mouse
- SwissProt: Q9JMJ4 Rat
- Unigene: 502705 Human
Anti-PRP19 antibody images:
Western blot - PRP19 antibody (ab27692)
All lanes : Anti-PRP19 antibody (ab27692) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 55 kDa
Observed band size : 55 kDa
Immunocytochemistry/ Immunofluorescence - PRP19 antibody (ab27692)
Ab27692, at 1/500 dilution, staining human PRP 19 in HeLa Cells by ICC/IF.
This image is part of an abreview submitted by Dr Kirk McManus
Western blot - PRP19 antibody (ab27692)
All lanes : Anti-PRP19 antibody (ab27692) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 55 kDa
Observed band size : 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRP19 antibody (ab27692)
Image courtesy of Human Protein Atlas. ab27692 staining PRP19 in human breast cancer, showing a distinct nuclear staining pattern. Paraffin embedded human breast cancer tissue was incubated with ab27692 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab27692 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
References for Anti-PRP19 antibody (ab27692)
This product has been referenced in:
- Sato Met al. Human PRP19 interacts with prolyl-hydroxylase PHD3 and inhibits cell death in hypoxia. Exp Cell Res 316:2871-82 (2010). WB, IP; Human.Read more (PubMed: 20599946) »
- Kleinridders Aet al. PLRG1 is an essential regulator of cell proliferation and apoptosis during vertebrate development and tissue homeostasis. Mol Cell Biol 29:3173-85 (2009). WB; Mouse.Read more (PubMed: 19307306) »
See all 2 publications for this product
Publishing research using ab27692? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"