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Anti-PRPF8 antibody [2834C1a] (ab51366) at 1/100 dilution + HeLa whole cell lysate at 25 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size : 273 kDa
Observed band size : 250 kDa (why is the actual band size different from the predicted?)
Anti-PRPF8 antibody [2834C1a] (ab51366) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size : 273 kDa
Observed band size : 250 kDa (why is the actual band size different from the predicted?)
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