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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Overview
Product name
Anti-PRPF8 antibody
See all PRPF8 products (3) ...
Description
Rabbit polyclonal to PRPF8
Tested applications
IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PRPF8.
(Peptide available as ab86623.)
Positive control
This antibody gave a positive signal in the following cell lysates: HeLa (Whole Cell lysate and Nuclear extract); NIH 3T3; A431; MEF1.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Sensory System >> Visual system
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 10 µg/ml
WB: Use at an assay dependent concentration. Detects a band of approximately 250 kDa (predicted molecular weight: 274 kDa).
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. Interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes.
Tissue specificity
Widely expressed.
Involvement in disease
Defects in PRPF8 are the cause of retinitis pigmentosa type 13 (RP13) [MIM:600059]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP13 inheritance is autosomal dominant.
Sequence similarities
Contains 1 MPN (JAB/Mov34) domain.
Domain
The MPN domain has structural similarity with viral ribonucleases and RNase H, but unlike RNases, it does not bind any metal ions.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus speckle.
Target information above from: UniProt accessionQ6P2Q9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 220 kDa U5 snRNP specific protein antibody
- 220 kDa U5 snRNP-specific protein antibody
- Apoptosis regulated protein 1 antibody
see all
Database links
- Entrez Gene: 10594 Human
- Entrez Gene: 192159 Mouse
- Omim: 607300 Human
- SwissProt: Q6P2Q9 Human
- SwissProt: Q9BW78 Human
- SwissProt: Q99PV0 Mouse
- Unigene: 181368 Human
- Unigene: 3757 Mouse
Anti-PRPF8 antibody images:
Western blot - PRPF8 antibody (ab79237)
All lanes : Anti-PRPF8 antibody (ab79237) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 274 kDa
Observed band size : 250 kDa (why is the actual band size different from the predicted?)
The 250 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to PRPF8.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRPF8 antibody (ab79237)
IHC image of PRPF8 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79237, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence - Anti-PRPF8 antibody (ab79237)
ICC/IF image of ab79237 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79237, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.
References for Anti-PRPF8 antibody (ab79237)
ab79237 has not yet been referenced specifically in any publications.
Publishing research using ab79237? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"