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Western blot - (ab87433)
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Immunocytochemistry/ Immunofluorescence - PRPF8 antibody (ab87433)
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Product Name
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PRPF8 antibody
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See all PRPF8 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PRPF8
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 2300 to the C-terminus of Human PRPF8.
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat, Cow
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 274 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following lysates: HeLa Whole Cell lysate; HeLa Nuclear Lysate; NIH 3T3 Whole Cell lysate; A431 Whole Cell Lysate; MEF1 Whole Cell Lysate.
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Cellular localization
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Nuclear; nuclear speckle
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Research areas
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Neuroscience >> Sensory System >> Visual system
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
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Relevance
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PRPF8 is a component of mammalian spliceosomes, large multiprotein complexes involved in the removal of introns from mRNA precursors. It is a central component of the spliceosome and may play a role in aligning the pre-mRNA 5' and 3' exons for ligation. PRPF8 is a component of both U2- and U12-dependent spliceosomes and it interacts with U5 snRNP proteins SNRP116 and WDR57/SPF38. Defects in PRPF8 are the cause of the autosomal dominant retinitis pigmentosa type 13 (RP13) characterised by degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As the condition progresses, far peripheral visual field is lost and eventually central vision as well.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.20 - 0.40 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PRPF8 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PRPF8 antibody images, references, products related to ab87433 and other tools.
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PRPF8 antibody images:
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 Western blot - (ab87433)
All lanes : PRPF8 antibody (ab87433) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 274 kDa
Observed band size : 250 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,175 kDa. We are unsure as to the identity of these extra bands.
The 250 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to PRPF8.
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Immunocytochemistry/ Immunofluorescence - PRPF8 antibody (ab87433)
ICC/IF image of ab87433 stained MCF-7 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87433, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) Hek293, and HepG2 cells at 1µg/ml.
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Search PubMed (MEDLINE) for references to PRPF8
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PRPF8 antibody - more information
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Customer reviews (feedback) regarding PRPF8 antibody |
Customer FAQs regarding PRPF8 antibody |
Protocols for PRPF8 antibody |
Price and availability of products related to PRPF8 antibody |
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PRPF8 antibody - related products:
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PRPF8 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PRPF8 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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