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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Overview
Product name
Anti-PRPF8 antibody
See all PRPF8 products (3) ...
Description
Rabbit polyclonal to PRPF8
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 2300 to the C-terminus of Human PRPF8.
(Peptide available as ab87434.)
Positive control
This antibody gave a positive signal in the following lysates: HeLa Whole Cell lysate; HeLa Nuclear Lysate; NIH 3T3 Whole Cell lysate; A431 Whole Cell Lysate; MEF1 Whole Cell Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Sensory System >> Visual system
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Applications
Show applications key
Our Abpromise guarantee covers the use of ab87433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 250 kDa (predicted molecular weight: 274 kDa).
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. Interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes.
Tissue specificity
Widely expressed.
Involvement in disease
Defects in PRPF8 are the cause of retinitis pigmentosa type 13 (RP13) [MIM:600059]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP13 inheritance is autosomal dominant.
Sequence similarities
Contains 1 MPN (JAB/Mov34) domain.
Domain
The MPN domain has structural similarity with viral ribonucleases and RNase H, but unlike RNases, it does not bind any metal ions.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus speckle.
Target information above from: UniProt accessionQ6P2Q9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 220 kDa U5 snRNP specific protein antibody
- 220 kDa U5 snRNP-specific protein antibody
- Apoptosis regulated protein 1 antibody
see all
Database links
- Entrez Gene: 10594 Human
- Entrez Gene: 192159 Mouse
- Entrez Gene: 287530 Rat
- Omim: 607300 Human
- SwissProt: Q6P2Q9 Human
- SwissProt: Q9BW78 Human
- SwissProt: Q99PV0 Mouse
- Unigene: 181368 Human
see all
Anti-PRPF8 antibody images:
Western blot - Anti-PRPF8 antibody (ab87433)
All lanes : Anti-PRPF8 antibody (ab87433) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 274 kDa
Observed band size : 250 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,175 kDa. We are unsure as to the identity of these extra bands.
The 250 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to PRPF8.
Immunocytochemistry/ Immunofluorescence - PRPF8 antibody (ab87433)
ICC/IF image of ab87433 stained MCF-7 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87433, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) Hek293, and HepG2 cells at 1µg/ml.
References for Anti-PRPF8 antibody (ab87433)
ab87433 has not yet been referenced specifically in any publications.
Publishing research using ab87433? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"