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Western blot - PSAP antibody (ab68466)
Anti-PSAP antibody (ab68466) at 1/500 dilution + HepG2 whole cell lysate
Predicted band size : 58 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence-PSAP antibody(ab68466)
ICC/IF image of ab68466 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68466, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
PSAP antibody for Western blot in Mouse (68466)
PSAP antibody for Western blot in Human (68466)
PSAP antibody for IHC-Fr in Rat (68466)
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