Anti-PSD95 antibody - Synaptic Marker (ab18258)

Thank you for the quick reply Jeremy. It was very helpful.

ANSWER:

I'm happy to help! Please let me know if you have any other questions.

To whom it may concern,
It's been a year since I conducted my last PSD-95 immuno experiment. I presently started back up again and
noticed the immunosignal went down a bit. This could be due to either degradation of pepsin enzyme (for antigen
retrieval) or of primary ab while in freezer storage for 1 yr. I believe the enzyme is more likely the cause of
the degradation. However, have you noticed any deterioration of PSD-95 immunosignal over 1 year? I aliquoted the
PSD-95 ab (ab18258 lot number: gr1360-1) into 5 ul aliquots and stored the aliquots at -20 degrees Celsius.
Thank you for your time,

ANSWER:

Thank you for your enquiry.

After storing this antibod in the freezer for 1 year it should still be fine.

You might try increasing the concentration of the primary two-fold to see if adding more antibody enhances the signal.

I hope this is helpful. Please contact us again if you have any further questions,.

I have a question about the Anti-PSD95 antibody - Synaptic Marker (ab18258): does this antibody recognize chick tissue? If not, are there any PSD95 antibody in your company reacts with chick? We would like to use the PSD95 antibody for immunostaining.

ANSWER:

Thank you very much for your interest in ab18258.

To our knowledge, ab18258 has not been tested in chicken and we do not have any anti-PSD95 antibodies that have been tested in chicken as far as we know. Therefore, I can offer a discount off a future purchase if you buy ab18258 now, test it in chicken and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 freeprimary antibody. If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab18258 in chicken. I will then send a discount code. This code must be issued before purchasing ab18258 so please wait for my reply before ordering.

2. Purchase ab18258 either by phone, fax, or online (www.abcam.com).

3. Test it in chicken.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab18258 turns out to be unsuitable for chicken, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Hi,

I have a customer who is after a suggestion of a good synapse marker for fluorescence IHC. They are labeling two groups of neurons in the rat brainstem, and need to confirm that they form a synapse.

I suggested Anti-PSD95 antibody - Synaptic Marker (http://www.abcam.com/PSD95-antibody-Synaptic-Marker-ab18258.html) to them, based on the fact that it is specific to rat, has been tested in IF and has 13 Abreviews and 11 publications.

The customer was indeed looking at Anti-PSD95 as well. More specifically, they are looking in the NTS at the level of the obex mostly and would like to know if this antibody will work in that region?

I look forward to your feedback.

Kind regards,

ANSWER:

Thank you for contacting us. I apologise for keeping you waiting.

Unfortunately this antibody has not been tested in this region, and therefore I cannot confirm it will work. However, if the antibody is used following the datasheet statements (working applications and species), it is covered by the 6 months Abpromise guarantee, and you will receive a replacement or reimbursement in case it doesn’t work.

There are other cell synapse markers that you may want to consider, as D2-protein, Synapsin, Synaptoporin or Vesicular glutamate transporters (VGLUT1 and VGLUT2).

I hope this helps and if I can assist further, please do not hesitate to contact me.

To whom it may concern,

I am the process of writing a paper and wanted to know what the PSD-95 antibody (ab18258) was raised against. Was it near the N-terminal end or C-terminal end of the PSD-95 mouse peptide?

ANSWER:

Thank you for contacting Abcam. The PSD95 antibody, ab18258 was generated to the N-terminal region of mouse. I hope the paper writing goes well and if you have any other questions please do not hesitate to contact us.