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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters
Overview
Product name
Anti-PSD95 antibody - Synaptic Marker
See all PSD95 products (15) ...
Description
Rabbit polyclonal to PSD95 - Synaptic Marker
Tested applications
IHC-FoFr, ICC/IF, WB, IHC-P, IHC-Fr, Sandwich ELISA, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Marmoset (common)
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse PSD95.
(Peptide available as ab18661.)
Positive control
ab18258 gave a positvie result in the following Tissue Lysates Mouse Brain Rat Brain
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 20874778)
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 - 3 µg/ml.Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IHC-P: Use at an assay dependent dilution.
IHC-Fr: 1/1000(PubMed: 17959791)
sELISA: Use at an assay dependent dilution. (PubMed: 20169337Used as detection antibody.)
IP: Use at an assay dependent concentration.
Target
Function
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
Tissue specificity
Brain.
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Domain
The PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to post-synaptic density.
Post-translational
modifications
Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Cell junction > synapse > synaptosome. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
Target information above from: UniProt accessionP78352
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
see all
Database links
- Entrez Gene: 1742 Human
- Entrez Gene: 13385 Mouse
- Entrez Gene: 29495 Rat
- Omim: 602887 Human
- SwissProt: P78352 Human
- SwissProt: Q62108 Mouse
- SwissProt: P31016 Rat
- Unigene: 463928 Human
see all
Anti-PSD95 antibody - Synaptic Marker images:
Western blot - PSD95 antibody - Synaptic Marker (ab18258)
All lanes : Anti-PSD95 antibody - Synaptic Marker (ab18258) at 1 µg/ml
Lane 1 : wildtype mouse brain tissue lysate
Lane 2 : PSD95 knockout mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size : 80 kDa
Observed band size : ~80 kDa (why is the actual band size different from the predicted?)
Immunoprecipitation - PSD95 antibody - Synaptic Marker (ab18258)
Lane 1 - Input lane 500ug mouse brain lysate
Lane 2 - IP lane 50ug mouse brain lysate
NB: as expected, an enrichment of PSD95 protein is observed in the IP lane.
Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
Western blot - PSD95 antibody - Synaptic Marker (ab18258)
All lanes : Anti-PSD95 antibody - Synaptic Marker (ab18258) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate (ab27253)
Lane 2 : Brain (Rat) Tissue Lysate (ab7942)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 80 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSD95 antibody - Synaptic Marker (ab18258)
ab18258 (1/1000) staining PSD9 in paraffin-embedded rat cerebellum. Tissue was fixed in formaldehyde, blocking performed using 1% BSA (10 mins/RT) and heat mediated antigen retrieval performed before staining. The secondary antibody (1/200) was goat anti rabbit IgG conjugated to Biotin. For further experimental details please refer to abreview.
Carl Hobbs, King`s College London, United Kingdom
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSD95 antibody - Synaptic Marker (ab18258)
ab18258 (1/500) staining PSD95 in paraffin-embedded Mouse Cerebellum (Top) and Medulla (Bottom) tissue, showing positive staining to the synaptic regions of the brain. Tissue was fixed in formaldehyde, blocking performed using 1% BSA (10 mins/RT) and heat mediated antigen retrieval performed before staining. The secondary antibody (1/200) was goat anti rabbit IgG conjugated to Biotin. For further experimental details please refer to abreview.
This image is courtesy of an abreview submitted by Carl Hobbs
Immunohistochemistry (Frozen sections) - Anti-PSD95 antibody - Synaptic Marker (ab18258)
ab18258 staining PSD95 in murine retinal tissue by Immunohistochemistry (Frozen sections).Tissue was fixed with paraformaldehyde, blocked using 10% serum for 30 minutes at 24°C, then incubated with ab18258 at a 1/1000 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.
Image courtesy of an anonymous Abreview.
Immunohistochemistry (Frozen sections) - Anti-PSD95 antibody - Synaptic Marker (ab18258)
ab18258 staining PSD95 in rat retinal tissue by Immunohistochemistry (Frozen sections).Tissue was fixed with paraformaldehyde, blocked using 10% serum for 30 minutes at 24°C, then incubated with ab18258 at a 1/1000 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.
Image courtesy of an anonymous Abreview.
Immunocytochemistry/ Immunofluorescence - Anti-PSD95 antibody - Synaptic Marker (ab18258)
ab18258 staining PSD95 in human SH-SY5Y cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, blocked with 10% serum for 20 minutes at 24°C, then incubated with ab18258 at a 1/1000 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-rabbit polyclonal used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).
Image courtesy of an anonymous Abreview.
Immunocytochemistry/ Immunofluorescence - PSD95 antibody - Synaptic Marker (ab18258)
ICC/IF image of ab18258 stained human SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab18258, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-PSD95 antibody - Synaptic Marker (ab18258)
This product has been referenced in:
- Cooke BM Synaptic reorganisation of the medial amygdala during puberty. J Neuroendocrinol 23:65-73 (2011). IHC-FoFr; Hamster.Read more (PubMed: 20874778) »
- Choi JGet al. Effects of optimized-SopungSunkiwon on memory impairment and enhancement. Neurosci Lett 491:93-8 (2011).Read more (PubMed: 21256184) »
See all 11 publications for this product
Publishing research using ab18258? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"