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ab12419 |
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ab12093 has been referenced in 7 publications.
Publishing research using ab12093? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Lane 1 : Anti-PSD95 antibody (ab12093) at 0.3 µg/ml Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
Lane 2 : Anti-PSD95 antibody (ab12093) at 1 µg/ml
Lanes 3 - 4 : Anti-PSD95 antibody (ab12093) at 3 µg/ml
Lane 1 : 10µg mouse brain extract/lane
Lane 2 : 10µg mouse brain extract/lane
Lane 3 : 10µg mouse brain extract/lane
Lane 4 : 10µg mouse brain extract/lane with
Performed under reducing conditions.
Predicted band size : 105 kDa
Observed band size : ~105 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 seconds
ab12093 PSD95 antibody immunostaining brain tissue (4% PFA perfused paraffin embedded 5 micron saggital section). ab12093 was used at 10 µg/ml for a 20 minute incubation at 37 degrees C (on a Ventana Discovery slide processor). Light antigen retrieval and then light protease treatment is recommended before addition of primary antibody ab12093 in this paradigm.
Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
ab12093 staining cultured rat primary hippocampal neurons by ICC/IF. The cultured neurons were fixed with 4% formaldehyde and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured neurons were then stained with ab12093 at 1/500 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 568 donkey anti-goat polyclonal antibody at 1/1000 was used as the secondary antibody. PSD95 expressed in exon, dendrites and particularly carrying in the dendritic spines
This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhury
All lanes : Anti-PSD95 antibody (ab12093) at 1/5000 dilution
Lane 1 : rat cortical lysate
Lane 2 : mouse cortical lysate
Lane 3 : BA21 human cortical lane lysate
Lane 4 : BA21 human cortical lane lysate
Lane 5 : BA21 human cortical lane lysate
Lane 6 : BA21 human cortical lane lysate
Lane 7 : BA21 human cortical lane lysate
Lane 8 : BA21 human cortical lane lysate
Lysates/proteins at 20 µg per lane.
Secondary
Alexafluor 680
Performed under reducing conditions.
Predicted band size : 105 kDa
See abreview for further details
This image is courtesy of an Abreview submitted by Dr Martin Broadstock
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