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Western blot - PSD95 antibody (ab12093)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSD95 antibody (ab12093)
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Western blot - PSD95 antibody (ab12093)
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Product Name
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PSD95 antibody
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See all PSD95 antibodies (13)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to PSD95
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Immunogen
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Synthetic peptide derived from residues 1 - 100 of Mouse PSD95.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-FrFl: 1/10 (PMID 19228947)
IHC-P: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately ~105 kDa (predicted molecular weight: ~105 kDa). Can be blocked with PSD95 peptide (ab12419) or PSD95 peptide (ab12419).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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mouse, rat and human brain (whole tissue and brain extract lysates)
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Cellular localization
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Cell Membrane and Cytoplasmic
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Research areas
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Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters
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Relevance
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PSD95 is a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with DLG2. With DLG2 it is recruited into the same NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.025% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PSD95 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PSD95 antibody images, references, products related to ab12093 and other tools.
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PSD95 antibody images:
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 Western blot - PSD95 antibody (ab12093)
Lane 1 : PSD95 antibody (ab12093) at 0.3 µg/ml
Lane 2 : PSD95 antibody (ab12093) at 1 µg/ml
Lanes 3 - 4 : PSD95 antibody (ab12093) at 3 µg/ml
Lane 1 : 10µg mouse brain extract/lane
Lane 2 : 10µg mouse brain extract/lane
Lane 3 : 10µg mouse brain extract/lane
Lane 4 : 10µg mouse brain extract/lane with PSD95 peptide (ab12419) at 1 µg/ml
Performed under reducing conditions.
Predicted band size : ~105 kDa
Observed band size : ~105 kDa
Exposure time : 10 seconds Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSD95 antibody (ab12093)
ab12093 PSD95 antibody immunostaining brain tissue (4% PFA perfused paraffin embedded 5 micron saggital section). ab12093 was used at 10 µg/ml for a 20 minute incubation at 37 degrees C (on a Ventana Discovery slide processor). Light antigen retrieval and then light protease treatment is recommended before addition of primary antibody ab12093 in this paradigm. Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
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Western blot - PSD95 antibody (ab12093)
All lanes : PSD95 antibody (ab12093) at 1/5000 dilution
Lane 1 : rat cortical lysate
Lane 2 : mouse cortical lysate
Lane 3 : BA21 human cortical lane lysate
Lane 4 : BA21 human cortical lane lysate
Lane 5 : BA21 human cortical lane lysate
Lane 6 : BA21 human cortical lane lysate
Lane 7 : BA21 human cortical lane lysate
Lane 8 : BA21 human cortical lane lysate
Lysates/proteins at 20 µg per lane.
Secondary
Alexafluor 680
Performed under reducing conditions.
Predicted band size : ~105 kDa
See abreview for further details This image is courtesy of an Abreview submitted by Dr Martin Broadstock
See Abreview
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References for PSD95 antibody (ab12093)
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This product has been used in: (two most recent references)
Koffie RM et al. Oligomeric amyloid beta associates with postsynaptic densities and correlates with excitatory synapse loss near senile plaques. Proc Natl Acad Sci U S A 106:4012-7 (2009). IHC-FrFl; Mouse. PubMed: 19228947
Oh EC et al. Transformation of cone precursors to functional rod photoreceptors by bZIP transcription factor NRL. Proc Natl Acad Sci U S A 104:1679-84 (2007). Mouse. PubMed: 17242361
See all 4 publication references for this product.
If you publish research using ab12093 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PSD95
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PSD95 antibody - more information
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Customer reviews (feedback) regarding PSD95 antibody |
Customer FAQs regarding PSD95 antibody |
Protocols for PSD95 antibody |
Price and availability of products related to PSD95 antibody |
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PSD95 antibody - related products:
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PSD95 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PSD95 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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