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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters
Overview
Product name
Anti-PSD95 antibody
See all PSD95 products (15) ...
Description
Goat polyclonal to PSD95
Tested applications
IHC-FrFl, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide derived from residues 1 - 100 of Mouse PSD95.
Positive control
mouse, rat and human brain (whole tissue and brain extract lysates)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FrFl: Use at an assay dependent concentration. (PubMed: 19228947)
WB: Use a concentration of 0.3 - 1 µg/ml.Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).Can be blocked with PSD95 peptide (ab12419).
IHC-P: Use a concentration of 10 µg/ml
Target
Function
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
Tissue specificity
Brain.
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Domain
The PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to post-synaptic density.
Post-translational
modifications
Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Cell junction > synapse > synaptosome. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
Target information above from: UniProt accessionP78352
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
see all
Database links
- Entrez Gene: 1742 Human
- Entrez Gene: 13385 Mouse
- Entrez Gene: 29495 Rat
- Omim: 602887 Human
- SwissProt: P78352 Human
- SwissProt: Q62108 Mouse
- SwissProt: P31016 Rat
- Unigene: 463928 Human
see all
Anti-PSD95 antibody images:
Western blot - PSD95 antibody (ab12093)
Lane 1 : Anti-PSD95 antibody (ab12093) at 0.3 µg/ml
Lane 2 : Anti-PSD95 antibody (ab12093) at 1 µg/ml
Lanes 3 - 4 : Anti-PSD95 antibody (ab12093) at 3 µg/ml
Lane 1 : 10µg mouse brain extract/lane
Lane 2 : 10µg mouse brain extract/lane
Lane 3 : 10µg mouse brain extract/lane
Lane 4 : 10µg mouse brain extract/lane with PSD95 peptide (ab12419) at 1 µg/ml
Performed under reducing conditions.
Predicted band size : 105 kDa
Observed band size : ~105 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 seconds
Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSD95 antibody (ab12093)
ab12093 PSD95 antibody immunostaining brain tissue (4% PFA perfused paraffin embedded 5 micron saggital section). ab12093 was used at 10 µg/ml for a 20 minute incubation at 37 degrees C (on a Ventana Discovery slide processor). Light antigen retrieval and then light protease treatment is recommended before addition of primary antibody ab12093 in this paradigm.
Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
Western blot - PSD95 antibody (ab12093)
All lanes : Anti-PSD95 antibody (ab12093) at 1/5000 dilution
Lane 1 : rat cortical lysate
Lane 2 : mouse cortical lysate
Lane 3 : BA21 human cortical lane lysate
Lane 4 : BA21 human cortical lane lysate
Lane 5 : BA21 human cortical lane lysate
Lane 6 : BA21 human cortical lane lysate
Lane 7 : BA21 human cortical lane lysate
Lane 8 : BA21 human cortical lane lysate
Lysates/proteins at 20 µg per lane.
Secondary
Alexafluor 680
Performed under reducing conditions.
Predicted band size : 105 kDa
See abreview for further details
This image is courtesy of an Abreview submitted by Dr Martin Broadstock
References for Anti-PSD95 antibody (ab12093)
This product has been referenced in:
- Proctor DTet al. Reduction in post-synaptic scaffolding PSD-95 and SAP-102 protein levels in the Alzheimer inferior temporal cortex is correlated with disease pathology. J Alzheimers Dis 21:795-811 (2010). WB; Human.Read more (PubMed: 20634587) »
- Koffie RMet al. Oligomeric amyloid beta associates with postsynaptic densities and correlates with excitatory synapse loss near senile plaques. Proc Natl Acad Sci U S A 106:4012-7 (2009). IHC-FrFl; Mouse.Read more (PubMed: 19228947) »
See all 6 publications for this product
Publishing research using ab12093? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"