If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab16496 for help.
There are no answered questions for ab16496
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Lane 1 : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 0.0825 µg/ml Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
Lanes 2 - 3 : Anti-PSD95 (phospho T19) antibody (ab16496) at 1 µg/ml
Lane 1 : mouse forebrain
Lane 2 : mouse forebrain
Lane 3 : mouse forebrain with
Lysates/proteins at 20 µg per lane.
Predicted band size : 100 kDa
Observed band size : 105 kDa (why is the actual band size different from the predicted?)
IHC image of PSD95 (Phospho T19) staining in human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16496, 1µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
1
Call 01223 696 000 or contact us