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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters
Overview
Product name
Anti-PSD95 (phospho T19) antibody
See all PSD95 products (15) ...
Description
Rabbit polyclonal to PSD95 (phospho T19)
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Human, Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse PSD95, phosphorylated at T19.
(Peptide available as ab17167.)
Positive control
mouse forebrain
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Phosphorylation of PSD95 dynamically regulates the clustering of PSD95/NMDA receptors at synapses, providing a possible mechanism for rapid changes in density and/or number of receptor at synapses.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/300
WB: Use a concentration of 1 µg/mlDetects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).Can be blocked with PSD95 peptide - phospho T19 (ab17167).
Target
Function
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
Tissue specificity
Brain.
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Domain
The PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to post-synaptic density.
Post-translational
modifications
Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Cell junction > synapse > synaptosome. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
Target information above from: UniProt accessionP78352
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
see all
Database links
- Entrez Gene: 1742 Human
- Entrez Gene: 13385 Mouse
- Entrez Gene: 29495 Rat
- Omim: 602887 Human
- SwissProt: P78352 Human
- SwissProt: Q62108 Mouse
- SwissProt: P31016 Rat
- Unigene: 463928 Human
see all
Anti-PSD95 (phospho T19) antibody images:
Western blot
Lane 1 : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 0.0825 µg/ml
Lanes 2 - 3 : Anti-PSD95 (phospho T19) antibody (ab16496) at 1 µg/ml
Lane 1 : mouse forebrain
Lane 2 : mouse forebrain
Lane 3 : mouse forebrain with PSD95 peptide - phospho T19 (ab17167) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size : 100 kDa
Observed band size : 105 kDa (why is the actual band size different from the predicted?)
Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSD95 (phospho T19) antibody (ab16496)
IHC image of PSD95 (Phospho T19) staining in human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16496, 1µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
References for Anti-PSD95 (phospho T19) antibody (ab16496)
ab16496 has not yet been referenced specifically in any publications.
Publishing research using ab16496? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"