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Product name
Anti-PSF2 antibody
See all PSF2 products (2) ...
Description
Rabbit polyclonal to PSF2
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide: MDAAEVEFLA EKELVTIIPN FSLDKIYLIG GDLGPFNPGL PVEVPLWLAI , corresponding to N terminal amino acids 1 -50 of Human PSF2
MDAAEVEFLAEKELVTIIPNFSLDKIYLIGGDLGPFNPGL PVEVPLWLAI
Positive control
Fetal muscle lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
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Western blot - PSF2 antibody (ab80816)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab80816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 21 kDa).Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Target
Relevance
The GINS complex is composed of four subunits, encoded by SLD5, PSF1, PSF2, and PSF3. In S. cerevisiae, it was first identified by genetic and biochemical methods to determine factors interacting with Sld5p. Genetic interactions between these four genes also suggest that they act together. The GINS complex was independently isolated in a large scale screen for cell cycle defects. A similar complex is found in Xenopus and has a ring-like structure. In yeast, all four genes are essential and cells defective in SLD5, PSF1, or PSF2 are impaired in their ability to replicate DNA. The complex localizes to origins of DNA replication and Sld5p was previously implicated as functioning in DNA replication due to its genetic interaction with DPB11. Additional genetic and biochemical interactions of the GINS complex with Dpb11p, Dpb2p, and Sld3p suggest that it functions in some way at the replication fork during DNA synthesis (1).
Cellular localization
Nuclear
Alternative names
- GINS complex subunit 2 antibody
- GINS2 antibody
- HSPC037 antibody
Database links
- Entrez Gene: 51659 Human
- Entrez Gene: 272551 Mouse
- Omim: 610609 Human
- SwissProt: Q9Y248 Human
- SwissProt: Q9D600 Mouse
- SwissProt: Q7ZT46 Xenopus laevis
Anti-PSF2 antibody images:
Western blot - PSF2 antibody (ab80816)
Anti-PSF2 antibody (ab80816) at 1 µg/ml + Fetal muscle lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at at 1/50000 dilution
Predicted band size : 21 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
References for Anti-PSF2 antibody (ab80816)
ab80816 has not yet been referenced specifically in any publications.
Publishing research using ab80816? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"