PSGL1 Human ELISA Kit (with color giving dyes) (ab119555)
Overview
- Product namePSGL1 Human ELISA Kit (with color giving dyes)
- Precision
Intra-assay Sample n Mean SD CV% Overall 3.2% - Tests1 x 96 test
- Sample typeCell culture supernatant, Serum
- Assay typeSandwich
- Sensitivity1 U/ml
- Range1.6 U/ml - 50 U/ml
- Recovery
94 %
Sample specific recovery Sample type Average % Range Serum 94 83% - 104% - Species reactivityReacts with: Human
- Product overview
ab119555 PSGL1 Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of Human PSGL1. ab119555 is for research use only. Not for diagnostic or therapeutic procedures.
Selectins are part of the broader family of cell adhesion molecules. The family of selectins consists of three structurally and functionally related molecules, L-selectin, P-selectin and E-selectin.
PSGL1 is found on white blood cells and endothelial cells and plays an important role in the recruitment of white blood cells into inflamed tissue. PSGL1 binds with the highest affinity to P-selectin.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Adhesive Films 4 units Assay Buffer Concentrate (20x) 1 x 5ml Biotin-Conjugate anti-human PSGL1 monoclonal antibody 1 x 100µl Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml Human PSGL1 Standard 2 vials Monoclonal anti-human PSGL1-coated microwell plate 1 unit Red-Dye 1 x 0.4ml Sample Diluent 1 x 50ml Stop Solution 1 x 15ml Streptavidin-HRP 1 x 150µl Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml - Research Areas
modificationsDisplays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
Target information above from: UniProt accession
Q14242
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CD162CD162 antigenCLA
- Cutaneous lymphocyte associated associated antigenP-selectin glycoprotein ligand 1PSGL 1PSGL-1Selectin P ligandSELPL_HUMANSELPLG
see all
- Entrez Gene: 6404 Human
- Omim: 600738 Human
- SwissProt: Q14242 Human
- Unigene: 591014 Human
Applications
Our Abpromise guarantee covers the use of ab119555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
PSGL1 Human ELISA Kit (with color giving dyes) images
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Sandwich ELISA - PSGL1 Human ELISA Kit (with color giving dyes) (ab119555)Representative standard curve using ab119555.
Protocols
References for PSGL1 Human ELISA Kit (with color giving dyes) (ab119555)
ab119555 has not yet been referenced specifically in any publications.
