Anti-PSMA antibody [EP3253] (ab76104)

Overview

• Product nameAnti-PSMA antibody [EP3253]See all PSMA primary antibodies ...
• Description
  Rabbit monoclonal [EP3253] to PSMA
• Tested applicationsWB, IP, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  A synthetic peptide corresponding to residues near the N terminus of human PSMA

• Positive controlLnCaP cell lysate.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP3253
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Amino acid metabolism

  • Cancer
  • Tumor biomarkers
  • Other

  • Signal Transduction
  • Metabolism
  • Amino Acids

Applications

Target

• FunctionHas both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
  Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
• Tissue specificityHighly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.
• Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
• DomainThe NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.
• Post-translational
  modificationsThe first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
  The N-terminus is blocked.
• Cellular localizationCytoplasm and Cell membrane.

Target information above from: UniProt accession Q04609 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2346 Human
  • Omim: 600934 Human
  • SwissProt: Q04609 Human
  • Unigene: 654487 Human

• Alternative names
  Cell growth inhibiting protein 27 antibodyCell growth-inhibiting gene 27 protein antibodyFGCP antibody

  Folate hydrolase (prostate-specific membrane antigen) 1 antibodyfolate hydrolase 1 antibodyfolate hydrolase antibodyFolate hydrolase prostate specific membrane antigen 1 antibodyFOLH 1 antibodyFOLH antibodyFOLH1 antibodyFOLH1_HUMAN antibodyfolylpoly gamma glutamate carboxypeptidase antibodyFolylpoly-gamma-glutamate carboxypeptidase antibodyGCP 2 antibodyGCP II antibodyGCP2 antibodyGCPII antibodyglutamate carboxylase II antibodyGlutamate carboxypeptidase 2 antibodyglutamate carboxypeptidase II antibodymembrane glutamate carboxypeptidase antibodymGCP antibodyN acetylated alpha linked acidic dipeptidase 1 antibodyN-acetylated-alpha-linked acidic dipeptidase I antibodyNAALAD 1 antibodyNAALAD1 antibodyNAALAdase antibodyNAALADase I antibodyprostate specific membrane antigen antibodyProstate specific membrane antigen variant F antibodyProstate-specific membrane antigen antibodyPSM antibodyPSMA antibodypteroylpoly gamma glutamate carboxypeptidase antibodyPteroylpoly-gamma-glutamate carboxypeptidase antibody

  see all

Anti-PSMA antibody [EP3253] images

• 

  Western blot - PSMA antibody [EP3253] (ab76104)
  Anti-PSMA antibody [EP3253] (ab76104) at 1/5000 dilution + LnCaP cell lysate at 10 µg
  
  Secondary
  HRP labelled goat anti-rabbit at 1/1000 dilution
  
  Predicted band size : 84 kDa
  Observed band size : 110 kDa (why is the actual band size different from the predicted?)