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ab4946
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab3330? Please let us know so that we can cite the reference in this datasheet
ab3330 has been referenced in 3 publications.
- Shen X et al. Processing of optineurin in neuronal cells. J Biol Chem 286:3618-29 (2011). WB, ICC/IF; Mouse. PubMed: 21059646
- Pérez-Galán P et al. Bortezomib resistance in mantle cell lymphoma is associated with plasmacytic differentiation. Blood 117:542-52 (2011). WB; Human. PubMed: 20956803
- Ahtoniemi T et al. Pyrrolidine dithiocarbamate inhibits induction of immunoproteasome and decreases survival in a rat model of amyotrophic lateral sclerosis. Mol Pharmacol 71:30-7 (2007). PubMed: 17008387
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSMB5 antibody (ab3330)
ab3330 (4µg/ml) staining PSMB5 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartment of the intestinal glands cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Proteasome 20S X antibody for WB in Mouse (3330)
PSMB5 antibody for Western blot in Human (3330)
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