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Western blot - PSMB5 antibody (ab78140)
Western blot - PSMB5 antibody (ab78140)
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Product Name 

PSMB5 antibody

 

See all PSMB5 antibodies (4)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PSMB5

Immunogen 

Synthetic peptide: AYGVMDRGYSYDLE conjugated to KLH, corresponding to amino acids 194-207 of Human PSMB5
BLAST 'AYGVMDRGYSYDLE' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Cow, Dog, Hrs, RMk, Orngt
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Predicted molecular weight: 23 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Proteasome

Relevance 

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP dependent proteolytic activity. PSMB5 (Proteasome subunit beta type 5) involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway.

 

Alternative names PSMB5 antibody (ab78140)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

534640    (Cow)
5693    (Human)
19173    (Mouse)
100173015    (Orangutan)
29425    (Rat)
677719    (Rhesus monkey)

Omim

    

600306    (Human)

SwissProt

    

Q32KL2    (Cow)
P28074    (Human)
O55234    (Mouse)
Q5R8S2    (Orangutan)
P28075    (Rat)

Unigene

    

422990    (Human)
2    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.01M Sodium Azide
Constituents: PBS, Whole serum

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PSMB5 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for PSMB5 antibody images, references, products related to ab78140 and other tools.

 

PSMB5 antibody images:

Product image  Western blot - PSMB5 antibody (ab78140)

Western blot - PSMB5 antibody (ab78140)

All lanes : PSMB5 antibody (ab78140) at 1/1000 dilution

Lane 1 : purified human erythrocyte derived 20S proteasome
Lane 2 : purified human erythrocyte derived 20S proteasome with PSMB5 blocking peptide
Lane 3 : HeLa S3 S100 cytosolic preparation
Lane 4 : HeLa S3 S100 cytosolic preparation with PSMB5 blocking peptide

Lysates/proteins at 25 µg per lane.


Predicted band size : 23 kDa

 
 

Search PubMed (MEDLINE) for references to PSMB5

 

PSMB5 antibody - more information

Customer reviews (feedback) regarding PSMB5 antibody

Customer FAQs regarding PSMB5 antibody

Protocols for PSMB5 antibody

Price and availability of products related to PSMB5 antibody

 

PSMB5 antibody - related products:

Products for Western blot

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Lysates

 

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Other PSMB5 Antibodies

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PSMB5 antibody - other tools:

Contact Abcam with a Technical Enquiry about PSMB5 antibody

 

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