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Western blot - PSMB5 antibody (ab78140)

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Product Name
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PSMB5 antibody
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See all PSMB5 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PSMB5
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Immunogen
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Synthetic peptide: AYGVMDRGYSYDLE conjugated to KLH, corresponding to amino acids 194-207 of Human PSMB5 BLAST 'AYGVMDRGYSYDLE' with or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Cow, Dog, Hrs, RMk, Orngt (Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: 1/1000. Predicted molecular weight: 23 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Proteasome
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Relevance
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The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP dependent proteolytic activity. PSMB5 (Proteasome subunit beta type 5) involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.01M Sodium Azide Constituents: PBS, Whole serum Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PSMB5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PSMB5 antibody images, references, products related to ab78140 and other tools.
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PSMB5 antibody images:
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Western blot - PSMB5 antibody (ab78140)
All lanes : PSMB5 antibody (ab78140) at 1/1000 dilution
Lane 1 : purified human erythrocyte derived 20S proteasome Lane 2 : purified human erythrocyte derived 20S proteasome with PSMB5 blocking peptide Lane 3 : HeLa S3 S100 cytosolic preparation Lane 4 : HeLa S3 S100 cytosolic preparation with PSMB5 blocking peptide
Lysates/proteins at 25 µg per lane.
Predicted band size : 23 kDa
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Search PubMed (MEDLINE) for references to PSMB5
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PSMB5 antibody - more information
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Customer reviews (feedback) regarding PSMB5 antibody |
Customer FAQs regarding PSMB5 antibody |
Protocols for PSMB5 antibody |
Price and availability of products related to PSMB5 antibody |
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PSMB5 antibody - related products:
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PSMB5 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PSMB5 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"