Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-PSMC5 antibody
See all PSMC5 products (6) ...
Description
Chicken polyclonal to PSMC5
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human, Pig
Immunogen
Synthetic peptide, corresponding to N terminal amino acids 2-160 of Human SUG1.
Positive control
E coli derived fusion protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Proteasome
-
Western blot - SUG1 antibody (ab37757)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/2000. Detects a band of approximately 70 kDa for the fusion protein (predicted molecular weight for the native protein: 46 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Human SUG1 (also known as p45, Rpt6, Cim3 and PSMC5) is a component of the 19S regulatory subunit of the 26S proteosome complex. It is one of the AAA ATPas found in the 19S regulatory complex that is thought to participate in the unfolding of ubiquitinated proteins in an ATP dependent manner. It has also been shown to directly interact with the nucleotide excision repair protein XPB.
Cellular localization
Cytoplasmic
Alternative names
- 26S protease regulatory subunit 8 antibody
- Cim3 antibody
- MSUG1 protein antibody
see all
Database links
- Entrez Gene: 282015 Cow
- Entrez Gene: 5705 Human
- Entrez Gene: 19184 Mouse
- Entrez Gene: 81827 Rat
- Omim: 601681 Human
- SwissProt: P62195 Human
- SwissProt: P62196 Mouse
- SwissProt: P62198 Rat
see all
Anti-PSMC5 antibody images:
Western blot - SUG1 antibody (ab37757)
Anti-PSMC5 antibody (ab37757) at 1/2000 dilution + E coli derived fusion protein at 2.5 µg
Secondary
Goat anti IgY HRP at 1/1000 dilution
Predicted band size : 46 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
The arrow next to the image points to the SUG1 fusion protein.
Observed band size may differ from predicted band size due to the test antigen being a fusion protein.
A colorimetric method was used for signal development.
References for Anti-PSMC5 antibody (ab37757)
ab37757 has not yet been referenced specifically in any publications.
Publishing research using ab37757? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"