Recombinant Anti-PSMD14 antibody [EPR4258] (ab109130)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4258] to PSMD14
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PSMD14 antibody [EPR4258]
See all PSMD14 primary antibodies -
Description
Rabbit monoclonal [EPR4258] to PSMD14 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, A431, K562, Namalwa, HeLa, and MCF7 cell lysates. IP: K-562 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4258 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
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IP |
1/30.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). |
IP
1/30. |
Target
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Function
Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear. -
Tissue specificity
Widely expressed. Highest levels in heart and skeletal muscle. -
Sequence similarities
Belongs to the peptidase M67A family. PSMD14 subfamily.
Contains 1 MPN (JAB/Mov34) domain. - Information by UniProt
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Database links
- Entrez Gene: 10213 Human
- Entrez Gene: 59029 Mouse
- Entrez Gene: 311078 Rat
- Omim: 607173 Human
- SwissProt: O00487 Human
- SwissProt: O35593 Mouse
- Unigene: 567410 Human
- Unigene: 218198 Mouse
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Alternative names
- 26S proteasome non-ATPase regulatory subunit 14 antibody
- 26S proteasome regulatory subunit rpn11 antibody
- 26S proteasome-associated PAD1 homolog 1 antibody
see all
Images
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All lanes : Anti-PSMD14 antibody [EPR4258] (ab109130) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Namalwa cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa -
PSMD14 was immunoprecipitated from 0.35 mg K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 µg with 109130 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 µg
Lane 2: ab109130 IP in K-562 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109130 in K-562 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab109130 has been referenced in 2 publications.
- Zhang Q et al. Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest. Brain Sci 12:N/A (2022). PubMed: 35884735
- Fletcher AJ et al. Trivalent RING Assembly on Retroviral Capsids Activates TRIM5 Ubiquitination and Innate Immune Signaling. Cell Host Microbe 24:761-775.e6 (2018). PubMed: 30503508