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Overview

  • Product namePSMD7 protein (Tagged-His Tag)See all PSMD7 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 53-324 (272 amino acids) of Human PSMD7 with an N terminal His tag. Predicted MWt: 32 kDa, UniProt ID: P51665
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 146 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesSFAVPFDEDDKDDSVWFLDHDYLENMYGMFKKVNARERIV GWYHTGPKLHKND IAINELMKRYCPNSVLVIIDVKPKD LGLPTEAYISVEEVHDDGTPTSKTFEHV TSEIGAEEAE EVGVEHLLRDIKDTTVGTLSQRITNQVHGLKGLNSKLLDI RSY LEKVATGKLPINHQIIYQLQDVFNLLPDVSLQEFV KAFYLKTNDQMVVVYLAS LIRSVVALHNLINNKIANRD AEKKEGQEKEESKKDRKEDKEKDKDKEKSDVKK EEKKE KK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91951 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        26S proteasome non ATPase regulatory subunit 726S proteasome non-ATPase regulatory subunit 726S proteasome regulatory subunit rpn8
        26S proteasome regulatory subunit S12MOV34Mov34 protein homologMOV34LP40Proteasome 26S S12Proteasome subunit p40PSD7_HUMANPSMD 7PSMD7S12
      see all
  • FunctionActs as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
  • Sequence similaritiesBelongs to the peptidase M67A family.
    Contains 1 MPN (JAB/Mov34) domain.

    • Target information above from: UniProt accession P51665 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    PSMD7 protein (Tagged-His Tag) images

    • SDS-PAGE - PSMD7 protein (Tagged-His Tag) (ab91951)
      SDS-PAGE - PSMD7 protein (Tagged-His Tag) (ab91951)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for PSMD7 protein (Tagged-His Tag) (ab91951)

    ab91951 has not yet been referenced specifically in any publications.

    Publishing research using ab91951 ? Please let us know so that we can cite the reference in this datasheet

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