PSME1 protein (Tagged-His Tag) (ab91718)
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Our Abpromise guarantee covers the use of ab91718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- 11S regulator complex alpha subunit11S regulator complex subunit alpha29 kD MCP activator subunit
- 29kD MCP activator subunitActivator of multicatalytic protease subunit 1IFI5111IGUP I-5111Interferon gamma IEF SSP 5111Interferon gamma inducible protein 5111Interferon gamma up regulated I 5111 proteinInterferon gamma up-regulated I-5111 proteinMGC8628PA28APA28alphaProteasome (prosome, macropain) activator subunit 1 (PA28 alpha)Proteasome activator 28 subunit alphaProteasome activator complex subunit 1Proteasome activator subunit 1PSME1PSME1_HUMANREG-alphaREGalpha
PSME1 protein (Tagged-His Tag) images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.
References for PSME1 protein (Tagged-His Tag) (ab91718)
ab91718 has not yet been referenced specifically in any publications.