Anti-PSPC1 antibody (ab104238)
Key features and details
- Rabbit polyclonal to PSPC1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PSPC1 antibody
See all PSPC1 primary antibodies -
Description
Rabbit polyclonal to PSPC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human PSPC1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab130811) -
Positive control
- This antibody gave a positive signal in both HEK293 + U20S whole cell lysates and HepG2 and MCF7 cell lines in IF.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 58 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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IP |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 58 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
IP
Use a concentration of 5 µg/ml. |
Target
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Function
Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers. -
Tissue specificity
Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart. -
Sequence similarities
Belongs to the PSPC family.
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleolus. Nucleus matrix. Cytoplasm. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in a RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps. - Information by UniProt
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Database links
- Entrez Gene: 55269 Human
- Entrez Gene: 66645 Mouse
- Entrez Gene: 305910 Rat
- Omim: 612408 Human
- SwissProt: Q8WXF1 Human
- SwissProt: Q8R326 Mouse
- SwissProt: Q4KLH4 Rat
- Unigene: 213198 Human
see all -
Alternative names
- Paraspeckle component 1 antibody
- Paraspeckle protein 1 antibody
- PSP1 antibody
see all
Images
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ab104238 staining PSPC1 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab104238 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-PSPC1 antibody (ab104238) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes -
PSPC1 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab104238.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 62kDa, non specific bands - 50 and 55kDa: We are unsure as to the identity of this extra band; PSPC1
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab104238 has been referenced in 5 publications.
- Wang X et al. Targeting the splicing factor NONO inhibits GBM progression through GPX1 intron retention. Theranostics 12:5451-5469 (2022). PubMed: 35910786
- Chen B et al. Live cell imaging and proteomic profiling of endogenous NEAT1 lncRNA by CRISPR/Cas9-mediated knock-in. Protein Cell 11:641-660 (2020). PubMed: 32458346
- Yu JSE et al. The FUS-DDIT3 Interactome in Myxoid Liposarcoma. Neoplasia 21:740-751 (2019). PubMed: 31220736
- Wang Z et al. NEAT1 modulates herpes simplex virus-1 replication by regulating viral gene transcription. Cell Mol Life Sci 74:1117-1131 (2017). WB, IF . PubMed: 27783096
- Shen W et al. 2'-Fluoro-modified phosphorothioate oligonucleotide can cause rapid degradation of P54nrb and PSF. Nucleic Acids Res 43:4569-78 (2015). PubMed: 25855809