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Anti-PSTAIR antibody [PSTAIR] (ab10345)

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Overview

Product name

Anti-PSTAIR antibody [PSTAIR]
See all PSTAIR products (2) ...

Description

Mouse monoclonal [PSTAIR] to PSTAIR

Tested applications

ELISA, IP, WB, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Cow, Human, Pig, Saccharomyces cerevisiae, Xenopus laevis, Monkey, Plants, Quail

Predicted to work with

Fish, a wide range of other species

Immunogen

Synthetic peptide: EGVPSTAIREISLLKE conjugated to BSA, corresponding to amino acids 42-57 of Human PSTAIR.

EGVPSTAIREISLLKE

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Epitope

Recognizes an epitope present in the PSTAIR sequence 1 of p34cdc2 (cdk1) and of other cyclin-dependent kinases containing the PSTAIR motif (cdk2 and cdk3).

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

PSTAIR

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other

  • Western blot - PSTAIR antibody [PSTAIR] (ab10345)Western blot - PSTAIR antibody [PSTAIR] (ab10345) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - PSTAIR antibody [PSTAIR] (ab10345)Immunocytochemistry/ Immunofluorescence - PSTAIR antibody [PSTAIR] (ab10345) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution (PMID 19187565).
IP: Use at an assay dependent dilution. It cannot precipitate p34cdc2 when it is complexed with cyclin B.
WB: 1/4000 (determined by indirect blotting using COS-7 cell extract). The antibody recognises 31-34kD proteins (1-4) bands.

Monoclonal Anti-PSTAIR may be used for the localization of cyclin-dependent kinases, containing the PSTAIR motif.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The cell cycle is controlled in part by cyclin dependent kinases (CDKs) which are themselves controlled by modifications such as phosphorylation of CDKs and formation of complex(es) with other proteins, including the cyclins. CDKs are key regulators of cell cycle progression. CDKs are closely related in size (35-40 kD) and sequence (>40% identity) and associate with and are activated by a cyclin which acts as a regulatory subunit. In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in the complex formation with cyclins. The availability of antibodies reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.

Alternative names

  • EGVPSTAIREISLLKE antibody
  • PSTAIR motif antibody
  • PSTAIR sequence antibody
  • PSTAIRE antibody

Anti-PSTAIR antibody [PSTAIR] images:

  Western blot - PSTAIR antibody [PSTAIR] (ab10345)

Western blot - PSTAIR antibody [PSTAIR] (ab10345)

All lanes : Anti-PSTAIR antibody [PSTAIR] (ab10345) at 1/6000 dilution

Lane 1 : Whole cell lysate from wild-type S. cerevisiae yeast cells
Lane 2 : Supernatant after incubation with Protein A-Sepharose beads
Lane 3 : Eluate from Protein A-Sepharose beads after treatment with TEV protease
Lane 4 : Product of boiling Protein A-Sepharose after TEV treatment

Secondary
Goat anti-mouse HRP conjugate
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 31 kDa
Observed band size : 32,34 kDa (why is the actual band size different from the predicted?)


Exposure time : 1 minute

This image is courtesy of an Abreview submitted by Mark Chee on 27 January 2006.

See Abreview

  Immunocytochemistry/ Immunofluorescence - PSTAIR antibody [PSTAIR] (ab10345)

Immunocytochemistry/ Immunofluorescence - PSTAIR antibody [PSTAIR] (ab10345)

ab10345 staining PSTAIR in Porcine Ooocytes by Immunocytochemistry/ Immunofluorescence. Oocytes were partially denuded by gentle pipettting in PEM-PVPc (80 mM PIPES, 5mM EGTA. 2 mM MgCl2, pH 6.8, supplemented with 0.3% (w/v) PVP) supplemented with either 0.01% (w/v) pronase (for < 22 h cultured oocytes), or 0.1% (w/v) hyaluronidase (for ≥ 22 h cultured oocytes). After washing in PEM-PVP at 37°C, oocytes were fixed in freshly prepared PEM-PVP containing 4% (v/v) paraformaldehyde at room temperature (RT) for 1 h. An Alexa Fluor® 488 conjugated Goat anti mouse IgG was used as secondary. ab10345 show green PSTAIR staining and DNA was stained blue. Arrow indicate the area that is shown enlarged in the inset. Left image show stainming with ab10345 and right one show staining with mouse IgG as negative control.

Image from Holzenspies J. et.al., BMC Dev Biol. 2009; 9: 8. (Fig. 1C).

References for Anti-PSTAIR antibody [PSTAIR] (ab10345)

This product has been referenced in:

  • Hoose SA  et al. A systematic analysis of cell cycle regulators in yeast reveals that most factors act independently of cell size to control initiation of division. PLoS Genet 8:e1002590 (2012). WB; Saccharomyces cerevisiae.Read more (PubMed: 22438835) »
  • Gotoh T  et al. Phosphorylation of Claspin is triggered by the nucleocytoplasmic ratio at the Xenopus laevis midblastula transition. Dev Biol 353:302-8 (2011). WB; Xenopus laevis.Read more (PubMed: 21396931) »

See all 7 publications for this product

Publishing research using ab10345? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"