For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Synthetic peptide: DGIVPDIAVGTKR, corresponding to N terminal amino acids 2-14 of Human PTBP1 .
Our Abpromise guarantee covers the use of ab5642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution. PubMed: 17967866|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60-65 kDa (predicted molecular weight: 61-64 kDa).Can be blocked with Human PTBP1 peptide (ab23105).|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"