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Anti-PTOV1 antibody (ab81173)

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If your product does not perform as described on this datasheet, we will refund or replace your product...

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Overview

Product name

Anti-PTOV1 antibody
See all PTOV1 products (2) ...

Description

Rabbit polyclonal to PTOV1

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow

Immunogen

Synthetic peptide corresponding to a region within the N terminal amino acids 109-158 (DSTAKLKRTLPCQAYVNQGENLETDQWPQKLIMQLIPQQ LLTTLGPLFRN) of Human PTOV1 (NP_059128).

DSTAKLKRTLPCQAYVNQGENLETDQWPQKLIMQLIPQQL LTTLGPLFRN

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Jurkat cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Cell cycle >> Cell division
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors

  • Western blot - PTOV1 antibody (ab81173)Western blot - PTOV1 antibody (ab81173) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab81173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

ELISA titre using peptide based assay 1/1562500.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

PTOV1 belongs to the Mediator complex subunit 25 family, PTOV1 subfamily. It may activate transcription and is required for nuclear translocation of FLOT1. PTOV1 promotes cell proliferation. There are three named isoforms.

Cellular localization

Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocates from the cytoplasm to the nucleus at the onset of S-phase. Also localizes to lipid rafts.

Alternative names

  • ACID2 antibody
  • Activator interaction domain containing protein 2 antibody
  • Gcap3 antibody
  • prostate tumor overexpressed 1 antibody
  • Prostate tumor overexpressed gene 1 antibody
  • PTOV 1 antibody
see all

Database links

Anti-PTOV1 antibody images:

  Western blot - PTOV1 antibody (ab81173)

Western blot - PTOV1 antibody (ab81173)

Anti-PTOV1 antibody (ab81173) at 1 µg/ml + Jurkat cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 47 kDa
Observed band size : 47 kDa
Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

Gel concentration 12%

References for Anti-PTOV1 antibody (ab81173)

This product has been referenced in:

  • Youn HS  et al. PTOV1 antagonizes MED25 in RAR transcriptional activation. Biochem Biophys Res Commun 404:239-44 (2011). WB; Human.Read more (PubMed: 21110951) »

See 1 publication for this product

Publishing research using ab81173? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"