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Overview

  • Product namePTP epsilon protein (Active)See all PTP epsilon proteins and peptides ...
  • Protein descriptionRecombinant fragment corresponding to aminoacids 75-700 of Human PTP epsilon; with a N-terminal proprietary tag; 91kDa calculated including tag. (BC050062; P23469).
  • Uniprot accessionP23469
  • Molecular weight91kDa inclusive of tags
  • Protein length626 amino acids
  • Expression hostBaculovirus infected sf9 cells

    • Properties

  • Purity>95% by SDS-PAGE
  • Biological activityThe specific activity of ab128555 was determined to be 8,600 nmol/min/mg.
  • E.C. Number3.1.3.48
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferpH: 7.50
    Constituents: 0.31% Glutathione, 0.02% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab128555 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution. Predicted molecular weight: 81 kDa.
    Functional Studies FuncS: Use at an assay dependent concentration.
    SDS-PAGE SDS-PAGE: Use at an assay dependent concentration.

    Protein info

    • Alternative names
        DKFZp313F1310FLJ57799FLJ58245
        HPTPEProtein tyrosine phosphatase epsilonProtein tyrosine phosphatase receptor type EProtein tyrosine phosphatase receptor type epsilonProtein tyrosine phosphatase receptor type epsilon polypeptideProtein-tyrosine phosphatase epsilonPTPEPtprePTPRE_HUMANR PTP EpsilonR-PTP-epsilonReceptor-type tyrosine-protein phosphatase epsilon
      see all
  • FunctionIsoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function.
    Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake.
    Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca(2+).
  • Tissue specificityExpressed in giant cell tumor (osteoclastoma rich in multinucleated osteoclastic cells).
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily.
    Contains 2 tyrosine-protein phosphatase domains.
  • DomainThe tyrosine-protein phosphatase 2 domain (D2) mediates dimerization. The extreme N- and C- termini of the D2 domain act to inhibit dimerization and removal of these sequences increases dimerization and inhibits enzyme activity.
  • Post-translational
    modifications    A catalytically active cytoplasmic form (p65) is produced by proteolytic cleavage of either isoform 1, isoform 2 or isoform 3.
    Isoform 1 and isoform 2 are phosphorylated on tyrosine residues by tyrosine kinase Neu.
    Isoform 1 is glycosylated.
  • Cellular localizationCytoplasm; Cell membrane and Cytoplasm. Predominantly cytoplasmic. A small fraction is also associated with nucleus and membrane. Insulin induces translocation to the membrane.

    • Target information above from: UniProt accession P23469 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    PTP epsilon protein (Active) images

    • SDS-PAGE - PTP epsilon protein (Active) (ab128555)
      SDS-PAGE - PTP epsilon protein (Active) (ab128555)
      SDS Page analysis of ab128555
    • Functional Studies - PTP epsilon protein (Active) (ab128555)
      Functional Studies - PTP epsilon protein (Active) (ab128555)
      The specific activity of ab128555 was determined to be 8,600 nmol/min/mg.

    References for PTP epsilon protein (Active) (ab128555)

    ab128555 has not yet been referenced specifically in any publications.

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