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Immunoprecipitation - PTP mu antibody [2C10] (ab23879)
![Immunoprecipitation - PTP mu antibody [2C10] (ab23879)](/ps/datasheet/images/ab23879.jpg)
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Product Name
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PTP mu antibody [2C10]
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See all PTP mu antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [2C10] to PTP mu
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Immunogen
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Recombinant extracellular domain fragment (Human).
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Reacts with
(species key)
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Ms
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Tested applications
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Flow Cyt, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at a concentration of 5 - 20 µg/ml.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Type 1 membrane protein
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
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Relevance
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PTP mu is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain, an Ig-like domain and four fibronectin type III-like repeats. This PTP has been shown to mediate cell-cell aggregation through the interaction with another molecule of this PTP on an adjacent cell. This PTP can interact with scaffolding protein RACK1/GNB2L1, which may be necessary for the downstream signaling in response to cell-cell adhesion.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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2C10
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Isotype
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IgG2b
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Light chain type
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kappa
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Purification notes
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Affinity chromatography on protein A/G beads.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PTP mu antibody [2C10] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PTP mu antibody [2C10] images, references, products related to ab23879 and other tools.
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PTP mu antibody [2C10] images:
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 Immunoprecipitation - PTP mu antibody [2C10] (ab23879)
SDS-PAGE analysis of anti-PTP mu immunoprecipitates. Lysates from 3T3[FL] cells labeled with [35S] methionine for 2 h were precipitated with: Lane 1: PTP mu rabbit polyclonal C-terminal ab23820
Lane C: control antibody (anti-GST)
Lanes 2-8: anti- PTP mu mAbs, including
Lane 5: 3D7 (ab23865)
Lane 8: 2C10 (ab23879) The mAbs precipitate both the uncleaved form of PTP mu (~200 kDa) as well as a cleaved form (100 kDa doublet). Note that the ab23879 formulation used to generate this image was hybridoma supernatant while the formulation available from Abcam has been purified. Martijn Gebbink, Univ Utrecht, Netherlands
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References for PTP mu antibody [2C10] (ab23879)
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This product has been used in:
Gebbink MF et al. Cell surface expression of receptor protein tyrosine phosphatase RPTP mu is regulated by cell-cell contact. J Cell Biol 131:251-60 (1995). PubMed: 7559782
If you publish research using ab23879 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PTP mu
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PTP mu antibody [2C10] - more information
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PTP mu antibody [2C10] - related products:
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Contact Abcam with a Technical Enquiry about PTP mu antibody [2C10] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunoprecipitation - PTP mu antibody [2C10] (ab23879)](/index.html?datasheet=23879#ab23879.jpg)
