![Immunoprecipitation - PTP mu antibody [2C10] (ab23879) image](#ab23879.jpg)
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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
Overview
Product name
Anti-PTP mu antibody [2C10]
See all PTP mu products (4) ...
Description
Mouse monoclonal [2C10] to PTP mu
Tested applications
Flow Cyt, WBmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Recombinant extracellular domain fragment (Human).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration
Concentration information loading...
Purification notes
Affinity chromatography on protein A/G beads.
Clonality
Monoclonal
Clone number
2C10
Isotype
IgG2b
Light chain type
kappa
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
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Immunoprecipitation - PTP mu antibody [2C10] (ab23879)
![Immunoprecipitation - PTP mu antibody [2C10] (ab23879) image](/ps/datasheet/Images/23/ab23879/ab23879.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab23879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use a concentration of 5 - 20 µg/ml.
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
Target
Function
Involved in cell-cell adhesion through homophilic interactions. May play a key role in signal transduction and growth control.
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 MAM domain.
Contains 2 tyrosine-protein phosphatase domains.
Cellular localization
Membrane.
Target information above from: UniProt accessionP28827
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hR PTPu antibody
- Protein tyrosine phosphatase mu antibody
- Protein-tyrosine phosphatase mu antibody
see all
Database links
- Entrez Gene: 19274 Mouse
- SwissProt: P28828 Mouse
- Unigene: 311809 Mouse
Anti-PTP mu antibody [2C10] images:
Immunoprecipitation - PTP mu antibody [2C10] (ab23879)
SDS-PAGE analysis of anti-PTP mu immunoprecipitates. Lysates from 3T3[FL] cells labeled with [35S] methionine for 2 h were precipitated with:
Lane 1: PTP mu rabbit polyclonal C-terminal ab23820
Lane C: control antibody (anti-GST)
Lanes 2-8: anti- PTP mu mAbs, including
Lane 5: 3D7 (ab23865)
Lane 8: 2C10 (ab23879)
The mAbs precipitate both the uncleaved form of PTP mu (~200 kDa) as well as a cleaved form (100 kDa doublet).
Note that the ab23879 formulation used to generate this image was hybridoma supernatant while the formulation available from Abcam has been purified.
Martijn Gebbink, Univ Utrecht, Netherlands
References for Anti-PTP mu antibody [2C10] (ab23879)
This product has been referenced in:
- Gebbink MFet al. Cell surface expression of receptor protein tyrosine phosphatase RPTP mu is regulated by cell-cell contact. J Cell Biol 131:251-60 (1995).Read more (PubMed: 7559782) »
See 1 publication for this product
Publishing research using ab23879? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"