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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab23820? Please let us know so that we can cite the reference in this datasheet
ab23820 has been referenced in 2 publications.
- Gebbink MF et al. Cell-cell adhesion mediated by a receptor-like protein tyrosine phosphatase. J Biol Chem 268:16101-4 (1993). PubMed: 8393854
- Gebbink MF et al. Cloning, expression and chromosomal localization of a new putative receptor-like protein tyrosine phosphatase. FEBS Lett 290:123-30 (1991). PubMed: 1655529
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - PTP mu antibody (ab23820)
All lanes : Anti-PTP mu antibody (ab23820) at 1/1000 dilution
Lane 1 : As above
Lane 2 : As above
Lane 3 : As above
Performed under reducing conditions.
Predicted band size : 163.6 kDa
Observed band size : 90,180 kDa (why is the actual band size different from the predicted?)
Immunoblot analysis of the PTP mu protein. Transiently transfected COS cells were analyzed for PTP mu expression.
Lane 1: lysates of cells tranfected with vector pMT2m without insert.
Lane 2: lysates of cells transfected with pMT2TR containing a truncated form of the hPTP mu cDNA.
Lane 3: lysates of cells transfected with pMT2mFL containing a full length mPTP mu cDNA.
Sizes of molecular mass markers are shown in kDa.
Antibody dilution: 1/1000
Image taken from Gebbink et al., 1991.
Martijn Gebbink, Univ Utrecht, Netherlands
Immunoprecipitation - PTP mu antibody (ab23820)
SDS-PAGE analysis of anti-PTP mu immunoprecipitates. Lysates from 3T3[FL] cells labeled with [35S] methionine for 2 h were precipitated with:
Lane 1: PTP mu rabbit polyclonal C-terminal ab23820
Lane C: control antibody (anti-GST),
Lanes 2-8: anti-PTP mu mAbs, including:
Lane 5, 3D7 (ab23865)
Lane 8, 2C10 (ab23879)
The antibodies precipitate both the uncleaved form of PTP mu (~200 kDa) as well as a cleaved form (100 kDa doublet).
Martijn Gebbink, Univ Utrecht, Netherlands
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