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I am not sure which order number you wish for me to provide - the original? |
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ANSWER: |
Yes, the original order number is required to process the free of charge replacement. |
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Thanks for your response. I'd be happy to try a different antibody. Pls let me know what I need to do to get it.Best regards |
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ANSWER: |
Could you please provide the purchase order number for me to send you the antibody? |
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no the issue was not resolved. we sent you the information requested but have not heard from you. |
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ANSWER: |
Thank you for your email. |
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no the issue was not resolved. we sent you the information requested but have not heard from you. |
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ANSWER: |
Thank you for your email. |
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Phonce call: ab23820 antibody fails in IP; Good results in WB. Good results in ICC/IF |
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ANSWER: |
According to our records, ab23820 was proving difficult to use inIP and we were in contact in order to help resolve the issue. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-PTP mu antibody (ab23820) at 1/1000 dilution
Lane 1 : As above
Lane 2 : As above
Lane 3 : As above
Performed under reducing conditions.
Predicted band size : 163.6 kDa
Observed band size : 90,180 kDa (why is the actual band size different from the predicted?)
Immunoblot analysis of the PTP mu protein. Transiently transfected COS cells were analyzed for PTP mu expression.
Lane 1: lysates of cells tranfected with vector pMT2m without insert.
Lane 2: lysates of cells transfected with pMT2TR containing a truncated form of the hPTP mu cDNA.
Lane 3: lysates of cells transfected with pMT2mFL containing a full length mPTP mu cDNA.
Sizes of molecular mass markers are shown in kDa.
Antibody dilution: 1/1000
Image taken from Gebbink et al., 1991.
Martijn Gebbink, Univ Utrecht, Netherlands
SDS-PAGE analysis of anti-PTP mu immunoprecipitates. Lysates from 3T3[FL] cells labeled with [35S] methionine for 2 h were precipitated with:
Lane 1: PTP mu rabbit polyclonal C-terminal ab23820
Lane C: control antibody (anti-GST),
Lanes 2-8: anti-PTP mu mAbs, including:
Lane 5, 3D7 (ab23865)
Lane 8, 2C10 (ab23879)
The antibodies precipitate both the uncleaved form of PTP mu (~200 kDa) as well as a cleaved form (100 kDa doublet).
Martijn Gebbink, Univ Utrecht, Netherlands
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