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Western blot - PTP mu antibody (ab23820)
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Immunoprecipitation - PTP mu antibody (ab23820)
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Product Name
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PTP mu antibody
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See all PTP mu antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PTP mu
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Immunogen
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Synthetic peptide: DQYKFCYEVALEYLNSG conjugated to BSA by a CDI linker, corresponding to C terminal amino acids 1436-1452 of Human PTP mu
BLAST 'DQYKFCYEVALEYLNSG' with  or 
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Reacts with
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Hu, Ms
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Tested applications
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 163.6 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Type 1 membrane protein
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
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Relevance
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PTP mu is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain, an Ig-like domain and four fibronectin type III-like repeats. This PTP has been shown to mediate cell-cell aggregation through the interaction with another molecule of this PTP on an adjacent cell. This PTP can interact with scaffolding protein RACK1/GNB2L1, which may be necessary for the downstream signaling in response to cell-cell adhesion.
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PTP mu antibody (ab23820) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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The antibody comes in the form of whole antiserum. In the original paper the antibody was referred to as Ab37.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PTP mu antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PTP mu antibody images, references, products related to ab23820 and other tools.
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PTP mu antibody images:
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 Western blot - PTP mu antibody (ab23820)
All lanes : PTP mu antibody (ab23820) at 1/1000 dilution
Lane 1 : As above
Lane 2 : As above
Lane 3 : As above
Performed under reducing conditions.
Predicted band size : 163.6 kDa
Observed band size : 90,180 kDa (why is the actual band size different from the predicted?)
Immunoblot analysis of the PTP mu protein. Transiently transfected COS cells were analyzed for PTP mu expression. Lane 1: lysates of cells tranfected with vector pMT2m without insert.
Lane 2: lysates of cells transfected with pMT2TR containing a truncated form of the hPTP mu cDNA.
Lane 3: lysates of cells transfected with pMT2mFL containing a full length mPTP mu cDNA. Sizes of molecular mass markers are shown in kDa.
Antibody dilution: 1/1000 Image taken from Gebbink et al., 1991. Martijn Gebbink, Univ Utrecht, Netherlands
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Immunoprecipitation - PTP mu antibody (ab23820)
SDS-PAGE analysis of anti-PTP mu immunoprecipitates. Lysates from 3T3[FL] cells labeled with [35S] methionine for 2 h were precipitated with: Lane 1: PTP mu rabbit polyclonal C-terminal ab23820
Lane C: control antibody (anti-GST),
Lanes 2-8: anti-PTP mu mAbs, including:
Lane 5, 3D7 (ab23865)
Lane 8, 2C10 (ab23879) The antibodies precipitate both the uncleaved form of PTP mu (~200 kDa) as well as a cleaved form (100 kDa doublet). Martijn Gebbink, Univ Utrecht, Netherlands
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References for PTP mu antibody (ab23820)
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This product has been used in: (two most recent references)
Gebbink MF et al. Cell-cell adhesion mediated by a receptor-like protein tyrosine phosphatase. J Biol Chem 268:16101-4 (1993). PubMed: 8393854
Gebbink MF et al. Cloning, expression and chromosomal localization of a new putative receptor-like protein tyrosine phosphatase. FEBS Lett 290:123-30 (1991). PubMed: 1655529
If you publish research using ab23820 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PTP mu
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PTP mu antibody - more information
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