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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
Overview
Product name
Anti-PTP mu antibody
See all PTP mu products (4) ...
Description
Rabbit polyclonal to PTP mu
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: DQYKFCYEVALEYLNSG conjugated to BSA by a CDI linker, corresponding to C terminal amino acids 1436-1452 of Human PTP mu
DQYKFCYEVA LEYLNSG
General notes
The antibody comes in the form of whole antiserum. In the original paper the antibody was referred to as Ab37.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
WB: 1/1000Detects a band of approximately 180 kDa (predicted molecular weight: 163.6 kDa).
Target
Function
Involved in cell-cell adhesion through homophilic interactions. May play a key role in signal transduction and growth control.
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 MAM domain.
Contains 2 tyrosine-protein phosphatase domains.
Cellular localization
Membrane.
Target information above from: UniProt accessionP28827
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hR PTPu antibody
- Protein tyrosine phosphatase mu antibody
- Protein-tyrosine phosphatase mu antibody
see all
Database links
- Entrez Gene: 5797 Human
- Entrez Gene: 19274 Mouse
- Omim: 176888 Human
- SwissProt: P28827 Human
- SwissProt: P28828 Mouse
- Unigene: 49774 Human
- Unigene: 311809 Mouse
Anti-PTP mu antibody images:
Western blot - PTP mu antibody (ab23820)
All lanes : Anti-PTP mu antibody (ab23820) at 1/1000 dilution
Lane 1 : As above
Lane 2 : As above
Lane 3 : As above
Performed under reducing conditions.
Predicted band size : 163.6 kDa
Observed band size : 90,180 kDa (why is the actual band size different from the predicted?)
Immunoblot analysis of the PTP mu protein. Transiently transfected COS cells were analyzed for PTP mu expression.
Lane 1: lysates of cells tranfected with vector pMT2m without insert.
Lane 2: lysates of cells transfected with pMT2TR containing a truncated form of the hPTP mu cDNA.
Lane 3: lysates of cells transfected with pMT2mFL containing a full length mPTP mu cDNA.
Sizes of molecular mass markers are shown in kDa.
Antibody dilution: 1/1000
Image taken from Gebbink et al., 1991.
Martijn Gebbink, Univ Utrecht, Netherlands
Immunoprecipitation - PTP mu antibody (ab23820)
SDS-PAGE analysis of anti-PTP mu immunoprecipitates. Lysates from 3T3[FL] cells labeled with [35S] methionine for 2 h were precipitated with:
Lane 1: PTP mu rabbit polyclonal C-terminal ab23820
Lane C: control antibody (anti-GST),
Lanes 2-8: anti-PTP mu mAbs, including:
Lane 5, 3D7 (ab23865)
Lane 8, 2C10 (ab23879)
The antibodies precipitate both the uncleaved form of PTP mu (~200 kDa) as well as a cleaved form (100 kDa doublet).
Martijn Gebbink, Univ Utrecht, Netherlands
References for Anti-PTP mu antibody (ab23820)
This product has been referenced in:
- Gebbink MFet al. Cell-cell adhesion mediated by a receptor-like protein tyrosine phosphatase. J Biol Chem 268:16101-4 (1993).Read more (PubMed: 8393854) »
- Gebbink MFet al. Cloning, expression and chromosomal localization of a new putative receptor-like protein tyrosine phosphatase. FEBS Lett 290:123-30 (1991).Read more (PubMed: 1655529) »
See all 2 publications for this product
Publishing research using ab23820? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"