Anti-PTP1B antibody [EP1837Y] (ab52650)

  Western blot - PTP1B antibody [EP1837Y] (ab52650)

Anti-PTP1B antibody [EP1837Y] (ab52650) at 1/200000 dilution + Jurkat cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size : 50 kDa
Observed band size : 50 kDa

  Immunohistochemistry (Paraffin-embedded sections) - PTP1B antibody [EP1837Y] (ab52650)

Immunohistochemical staining of paraffin-embedded human tonsils using ab52650 at a 1/100 dilution.

  Immunocytochemistry/ Immunofluorescence - PTP1B antibody [EP1837Y] (ab52650)

ICC/IF image of ab52650 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52650, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Flow Cytometry-PTP1B antibody [EP1837Y](ab52650)

Overlay histogram showing HepG2 cells stained with ab52650 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52650, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.