Anti-PRL-1 antibody (ab121185)
Key features and details
- Rabbit polyclonal to PRL-1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRL-1 antibody
See all PRL-1 primary antibodies -
Description
Rabbit polyclonal to PRL-1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PRL-1 aa 23-156.
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Positive control
- Human placenta tissue; Transfected HEK293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/250 - 1/500.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/250 - 1/500. |
Target
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Function
Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. May play a role in the development and maintenance of differentiating epithelial tissues. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. -
Tissue specificity
Expressed in bone marrow, lymph nodes, T lymphocytes, spleen, thymus and tonsil. Overexpressed in tumor cell lines. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family.
Contains 1 tyrosine-protein phosphatase domain. -
Developmental stage
Expressed in fetal liver. -
Post-translational
modificationsFarnesylated. Farnesylation is required for membrane targeting. Unfarnesylated forms are shifted into the nucleus. -
Cellular localization
Cell membrane. Early endosome. Endoplasmic reticulum. Cytoplasm. Cytoplasm > cytoskeleton > spindle. And mitotic spindle. - Information by UniProt
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Database links
- Entrez Gene: 7803 Human
- Omim: 601585 Human
- SwissProt: Q93096 Human
- Unigene: 227777 Human
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Alternative names
- HH 72 antibody
- HH72 antibody
- Hypothetical protein DKFZp779M0721 antibody
see all
Images
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ab121185, at 1/75 dilution, staining PRL-1 in paraffin-embedded Human placenta tissue, by Immunohistochemistry.
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All lanes : Anti-PRL-1 antibody (ab121185) at 1/250 dilution
Lane 1 : Negative control (vector-only transfected HEK293T lysate)
Lane 2 : PRL-1-transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab121185 has been referenced in 2 publications.
- Hu H et al. GINS2 regulates the proliferation and apoptosis of colon cancer cells through PTP4A1. Mol Med Rep 25:N/A (2022). PubMed: 35137928
- Chen M et al. Long non-coding RNA USP30-AS1 aggravates the malignant progression of cervical cancer by sequestering microRNA-299-3p and thereby overexpressing PTP4A1. Oncol Lett 22:505 (2021). PubMed: 33986866