![Immunohistochemistry (Paraffin-embedded sections) - PTPN13 antibody [AC21] (ab49446) image](#fas.bmp)
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Product name
Anti-PTPN13 antibody [AC21]
See all PTPN13 products (4) ...
Description
Mouse monoclonal [AC21] to PTPN13
Specificity
Human PTPN13 protein.
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Prokaryotic recombinant fusion protein corresponding to N terminal amino acids 239-342 of Human PTPN13 (Fas-associated phosphatase 1).
Positive control
Tonsil and normal human thyroid.
General notes
Staining pattern is mainly cytoplasmic with occasional nuclear staining observed.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
AC21
Myeloma
P3-NS1/1-Ag4-1
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Phosphatases
Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
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Immunohistochemistry (Paraffin-embedded sections) - PTPN13 antibody [AC21] (ab49446)
![Immunohistochemistry (Paraffin-embedded sections) - PTPN13 antibody [AC21] (ab49446) image](/ps/datasheet/Images/49/ab49446/fas.bmp)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab49446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/40 - 1/80. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Incubate with primary antibody for 60 minutes at 25°C.
Target
Relevance
PTPN13 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP is a large protein that possesses a PTP domain at C-terminus, and multiple noncatalytic domains, which include a domain with similarity to band 4.1 superfamily of cytoskeletal associated proteins, a region consisting of five PDZ domains, and a leucine zipper motif. This PTP was found to interact with, and dephosphorylate Fas receptor, as well as I-kappa-B-alpha through the PDZ domains, which suggested its role in Fas mediated programmed cell death. This PTP was also shown to interact with GTPase-activating protein, and thus may function as a regulator of Rho signaling pathway.
Cellular localization
Cytoplasmic
Alternative names
- APO1/CD95 (Fas) associated phosphatase antibody
- Fas associated phosphatase 1 antibody
- Protein tyrosine phosphatase non receptor type 13 antibody
see all
Database links
- Entrez Gene: 5783 Human
- Omim: 600267 Human
- SwissProt: Q12923 Human
- Unigene: 436142 Human
- Unigene: 643586 Human
Anti-PTPN13 antibody [AC21] images:
Immunohistochemistry (Paraffin-embedded sections) - PTPN13 antibody [AC21] (ab49446)
Immunohistochemical staining (Paraffin section) for PTPN13 (Fas-associated phosphatase 1) using ab49446 with normal Human thyroid as positive control. Note intense cytoplasmic staining of thyroid epithelial cells.
References for Anti-PTPN13 antibody [AC21] (ab49446)
This product has been referenced in:
- Glondu-Lassis M et al. PTPL1/PTPN13 regulates breast cancer cell aggressiveness through direct inactivation of Src kinase. Cancer Res 70:5116-26 (2010). IHC-P; Human.Read more (PubMed: 20501847) »
See 1 publication for this product
Publishing research using ab49446? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"