Anti-PUMA antibody - C-terminal (ab227130)
Key features and details
- Rabbit polyclonal to PUMA - C-terminal
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PUMA antibody - C-terminal
See all PUMA primary antibodies -
Description
Rabbit polyclonal to PUMA - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PUMA (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q9BXH1 -
Positive control
- WB: HCT 116 treated with cisplatin whole cell lysate; K562 and THP-1 whole cell extracts. IP: HCT 116 treated with cisplatin whole cell extract. IHC-P: Human hepatoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
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IP |
1/100 - 1/500.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 21 kDa. |
IP
1/100 - 1/500. |
Target
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Function
Essential mediator of p53-dependent and p53-independent apoptosis. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the Bcl-2 family. -
Cellular localization
Mitochondrion. Localized to the mitochondria in order to induce cytochrome c release. - Information by UniProt
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Database links
- Entrez Gene: 27113 Human
- Omim: 605854 Human
- SwissProt: Q9BXH1 Human
- Unigene: 467020 Human
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Alternative names
- BBC 3 antibody
- Bbc3 antibody
- BBC3_HUMAN antibody
see all
Images
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All lanes : Anti-PUMA antibody - C-terminal (ab227130) at 1/1000 dilution
Lane 1 : HCT 116 (human colorectal carcinoma cell line) with mock treatment for 0 hours, whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) with 30 uM cisplatin treatment for 24 hours, whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 21 kDa15% SDS-PAGE gel.
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All lanes : Anti-PUMA antibody - C-terminal (ab227130) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 21 kDa12 % SDS-PAGE gel.
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PUMA was immunoprecipitated from HCT 116 (human colorectal carcinoma cell line) treated with 30 µM cisplatin for 24 hours, whole cell extract with 4 µg of ab227130. Western blot was performed from the immunoprecipitate using ab227130. Anti-rabbit IgG was used as a secondary reagent.
Lane 1: Preimmune Rabbit IgG instead of ab227130 in HCT 116 treated with 30 µM cisplatin for 24 hours, whole cell extract.
Lane 2: ab227130 IP in HCT 116 treated with 30 µM cisplatin for 24 hours, whole cell extract.
15 % SDS-PAGE gel.
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Paraffin-embedded human hepatoma tissue tissue stained for PUMA using ab227130 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227130 has not yet been referenced specifically in any publications.