Anti-PYCR2 antibody (ab103535)
Key features and details
- Rabbit polyclonal to PYCR2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PYCR2 antibody -
Description
Rabbit polyclonal to PYCR2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human PYCR2 aa 1-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- HeLa cell lysate; PYCR2 transfected 293T cell line lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab103535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/500 - 1/1000. Predicted molecular weight: 34 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 34 kDa. |
Target
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Function
Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH. -
Tissue specificity
Detected in erythrocytes (at protein level). -
Pathway
Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1. -
Sequence similarities
Belongs to the pyrroline-5-carboxylate reductase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 29920 Human
- SwissProt: Q96C36 Human
- Unigene: 654718 Human
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Alternative names
- 1810018M05Rik antibody
- FLJ54750 antibody
- Leftb antibody
see all
Images
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All lanes : Anti-PYCR2 antibody (ab103535) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate- 18µg
Lane 2 : HeLa whole cell lysate- 36µg
Lane 3 : RPE-1 whole cell lysate- 18µg
Lane 4 : RPE-1 whole cell lysate- 36µg
Secondary
All lanes : LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 7 minutesBlocking: 10% Milk
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Anti-PYCR2 antibody (ab103535) at 1/500 dilution + HeLa cell lysate at 50 µg
Predicted band size: 34 kDa -
All lanes : Anti-PYCR2 antibody (ab103535) at 1/500 dilution
Lane 1 : PYCR2 transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 34 kDa
Datasheets and documents
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Datasheet download
References (3)
ab103535 has been referenced in 3 publications.
- Oudaert I et al. Pyrroline-5-Carboxylate Reductase 1: a novel target for sensitizing multiple myeloma cells to bortezomib by inhibition of PRAS40-mediated protein synthesis. J Exp Clin Cancer Res 41:45 (2022). PubMed: 35105345
- Yin F et al. Pyrroline-5-Carboxylate Reductase-2 Promotes Colorectal Cancer Progression via Activating PI3K/AKT/mTOR Pathway. Dis Markers 2021:9950663 (2021). PubMed: 34512817
- Keeratichamroen S et al. Identification of potential cervical cancer serum biomarkers in Thai patients. Oncol Lett 19:3815-3826 (2020). PubMed: 32391095