Anti-Pancreatic alpha amylase antibody (ab231119)
Key features and details
- Rabbit polyclonal to Pancreatic alpha amylase
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Pancreatic alpha amylase antibody
See all Pancreatic alpha amylase primary antibodies -
Description
Rabbit polyclonal to Pancreatic alpha amylase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human, Pig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Rat Pancreatic alpha amylase aa 200-500. Expressed in E. coli.
Database link: G3V844 -
Positive control
- IHC-P: Rat pancreas tissue. WB: Recombinant rat Pancreatic alpha amylase protein. Rat pancreas lysate and serum.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. |
Target
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Relevance
Pancreatic amylase is a digestive enzyme secreted by the pancreas. It consists of a single polypeptide chain with a molecular weight of 54 kDa. Pancreatic amylase occurs in serum at a concentration of about 60µg/L, from a total amylase concentration in serum of about 120µg/L. Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. Measurement of amylase concentrations in serum is a diagnostic test for various pancreatic disorders (especially acute and relapsing pancreatitis). -
Cellular localization
Secreted protein; extracellular space. -
Database links
- Entrez Gene: 279 Human
- Entrez Gene: 280 Human
- Entrez Gene: 545562 Mouse
- Entrez Gene: 397397 Pig
- Omim: 104650 Human
- Omim: 104660 Human
- SwissProt: P04746 Human
- SwissProt: P19961 Human
see all -
Alternative names
- 1 4 alpha D glucan glucanohydrolase antibody
- 1,4 alpha D glucan glucanohydrolase 2B antibody
- Alpha amylase 2B antibody
see all
Images
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Formalin-fixed, paraffin-embedded rat pancreas tissue stained for Pancreatic alpha amylase with ab231119 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-Pancreatic alpha amylase antibody (ab231119) at 2 µg/ml + Rat pancreas lysate
Developed using the ECL technique. -
Anti-Pancreatic alpha amylase antibody (ab231119) at 2 µg/ml + Rat serum
Developed using the ECL technique. -
Anti-Pancreatic alpha amylase antibody (ab231119) at 2 µg/ml + Recombinant rat Pancreatic alpha amylase protein
Developed using the ECL technique.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231119 has not yet been referenced specifically in any publications.