Anti-Parainfluenza Virus type 1 nucleocapsid antibody [7F5] (ab34752)
Key features and details
- Mouse monoclonal [7F5] to Parainfluenza Virus type 1 nucleocapsid
- Suitable for: ELISA, WB
- Isotype: IgG1
Overview
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Product name
Anti-Parainfluenza Virus type 1 nucleocapsid antibody [7F5] -
Description
Mouse monoclonal [7F5] to Parainfluenza Virus type 1 nucleocapsid -
Host species
Mouse -
Specificity
This antibody reacts with nucleocapsid protein of human parainfluenza virus type 1 (hPIV-1). It does not cross-react with nucleocapsid proteins of Sendai virus and human parainfluenza virus type 3 (hPIV-3), neither does it react with nucleocapsid proteins of measles, mumps, Menangle, Nipah, and Hendra viruses. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Human respirovirus 1 -
Immunogen
Recombinant full length protein corresponding to Parainfluenza Virus type 1 nucleocapsid.
Database link: Q8QT33 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
7F5 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/1000 - 1/10000.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 58 kDa.
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Notes |
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ELISA
1/1000 - 1/10000. |
WB
1/1000 - 1/5000. Predicted molecular weight: 58 kDa. |
Target
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Relevance
Human parainfluenza viruses (HPIV) were first discovered in the late 1950s. HPIV is genetically and antigenically divided into types 1 to 4. HPIV 1 to HPIV 3 are major causes of lower respiratory infections in infants, young children, the immunocompromised, the chronically ill, and the elderly. Each subtype can cause somewhat unique clinical diseases in different hosts. HPIV are enveloped and of medium size (150 to 250 nm), and their RNA genome is in the negative sense. These viruses belong to the Paramyxoviridae family, one of the largest and most rapidly growing groups of viruses causing significant human and veterinary disease. HPIV are closely related to recently discovered megamyxoviruses (Hendra and Nipah viruses) and metapneumovirus. Parainfluenza viruses are enveloped viruses with a negative strand, ~15 500 nucleotide-long nonsegmented RNA genome which encodes two envelope glycoproteins, the haemagglutinin-neuraminidase (HN), and the fusion protein (F, itself cleaved into F1 and F2 subunits), a matrix protein (M), a nucleocapsid protein (N) and several nonstructural proteins including the viral replicase (L). -
Cellular localization
Cytoplasmic -
Alternative names
- N antibody
- NP antibody
- Nucleocapsid protein antibody
see all
Images
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Immunoblot of recombinant nucleocapsid protein (NP) of human parainfluenza virus type 1 (hPIV-1) ab34752 at a concentration of 1 µg/ml.
MW markers;
lane 1: crude yeast cell lysate (mock control)
Lane 2: crude lysate of transformed yeast cells expressing NP of hPIV-1;
lane 3: purified recombinant NP of hPIV-1;
lane 4: purified recombinant NP of Sendai virus;
lane 5: lysate with recombinant NP of hPIV-3;
lanes 6-8: lysates with recombinant NP of Menangle (6), Hendra (7) and Nipah (8) viruses.
Panel A : SDS-PAGE, Coomasie staining, B: immunoblot with ab34752.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab34752 has not yet been referenced specifically in any publications.