Recombinant Anti-Parathyroid Hormone antibody [EPR20598] (ab213557)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20598] to Parathyroid Hormone
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Parathyroid Hormone antibody [EPR20598]
See all Parathyroid Hormone primary antibodies -
Description
Rabbit monoclonal [EPR20598] to Parathyroid Hormone -
Host species
Rabbit -
Specificity
ab213557 has non-specific staining on mouse and rat kidney
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Tested applications
Suitable for: IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Hamster -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC: Mouse parathyroid, rat parathyroid IHC (Frozen): Mouse and rat parathyroid tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20598 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
1/100.
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Notes |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/100. |
Target
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Function
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells. -
Involvement in disease
Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism. -
Sequence similarities
Belongs to the parathyroid hormone family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 19226 Mouse
- Entrez Gene: 24694 Rat
- SwissProt: P04089 Rat
- Unigene: 9768 Rat
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Alternative names
- hPTH antibody
- Parathormone antibody
- Parathyrin antibody
see all
Images
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat parathyroid tissue labeling Parathyroid Hormone with ab213557 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Granular cytoplasmic staining on chief cells of rat parathyroid is observed, no staining on adjacent thyroid cells (PMID: 7615802). The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse parathyroid tissue labeling Parathyroid Hormone with ab213557 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Granular cytoplasmic staining on chief cells of mouse parathyroid is observed, no staining on adjacent thyroid cells (PMID: 7615802). The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded rat parathyroid tissue labeling Parathyroid Hormone with ab213557 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051 ready to use). Granular cytoplasmic staining on chief cells of rat parathyroid is observed, no staining on adjacent thyroid cells (PMID: 7615802). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse parathyroid tissue labeling Parathyroid Hormone with ab213557 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051 ready to use). Granular cytoplasmic staining on chief cells of mouse parathyroid is observed, no staining on adjacent thyroid cells (PMID: 7615802). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab213557 has not yet been referenced specifically in any publications.