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Anti-Parathyroid Hormone antibody (ab53040)

Submit an Abreview Q&A (8)

Overview

  • Product nameAnti-Parathyroid Hormone antibodySee all Parathyroid Hormone primary antibodies ...
  • Description
    Rabbit polyclonal to Parathyroid Hormone
  • SpecificityThis antibody detects endogenous levels of total parathyroid hormone
  • Tested applicationsSandwich ELISA, WB, ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from human Parathyroid Hormone corresponding to amino acid 71 to 100.

  • Positive controlA549 cell extract

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°C
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab53040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA sELISA: Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [A1/70] to Parathyroid Hormone (ab14493). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab14493 as Capture.
WB WB: 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 13 kDa).
ELISA ELISA: 1/10000.

Target

  • FunctionPTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • Involvement in diseaseDefects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
  • Sequence similaritiesBelongs to the parathyroid hormone family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P01270 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        hPTH antibodyParathormone antibodyParathyrin antibody
        Parathyroid hormone 1 antibodyParathyroid hormone antibodyPTH antibodyPTH1 antibodyPTH1 receptor antibodyPTH1R antibodyPTHR antibodyPTHR1 antibodyPTHY_HUMAN antibody
      see all

    Anti-Parathyroid Hormone antibody images

    Protocols

    References for Anti-Parathyroid Hormone antibody (ab53040)

    ab53040 has not yet been referenced specifically in any publications.

    Publishing research using ab53040 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab53040 Submit a Question for ab53040

    Displaying 1 - 8 of 8 results for Abreviews and Q&A

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    thanks for replying, . Could you please specify whether this antibody pair is able to recognize the PTH-derived peptide 1-34 since i'm interested in measuring plasma levels of this peptide. It's clear from datasheet that the ab 14493 is specific for the P...

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    Thank you for your reply.

    The amino acid range of the immunogen for ab53040 is aa50-100 of human parathyroid hormone with accession number P01270 (http://www.uniprot.org/uniprot/P01270).

    If you could provide the exact protein sequence ...

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    In the product information of ab53040, the exactly description of the inmunogen is: Synthetic peptide derived from human Parathyroid.
    Taking in account that in the inmunogen description in ab40630 describes exactly the sequence of the synthetic peptid...

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    Thank you for your email.

    We generally add the location of immunogen e.g. N-terminal, C-terminal or internal on datasheet however I do agree this wasn't the case for this ab53040. This antibody would pick up the whole PTH but not 1-34 aa peptide....

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    To whom it may concern:

    I have two antibodies against human PTH from Abcam :

    ab53040
    ab40630

    and I´m using them in Western Blot and Dot Blot assays
    to identify PTH (1-34), this means a protein with only the first ...

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    Thank you for your email.

    The immunogen of antibody ab53040 is a sequence from amino acid 71 to aa 100 which might be the reason this antibody is not detecting band of interest.

    ab40630 is indeed tested for 100% reactivity with 1-34 pept...

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    Thanks for your efforts to make things work, I see it as our both interest to succeed with it.
    I'm very curious about your lab answer about ab53040, please let me know when you get their answer.
    I really hope it will work, we are looking for goo...

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    Thank you very much for your reply and for the information.
    I have added a vial of ab40630 to a shipment and forwarded the details of this replacement to your contact.
    I will be back in touch regarding ab53040, and if there is anything else that ...

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    Thanks for your answer.
    I think we'll try the ab40630 and hope for good...
    Indeed, we order Abcam antibodies through Zotal.
    You are right about the PTHrP MW, and attached you'll find two papers for example about the PTHrP expression in A549...

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    Thank you very much for your reply and for sending the information about the PTHrP expression in A549 cells.
    I will follow-up with the lab about ab53040, and I am happy to send a vial of ab40630. I will need to send this vial first to Zotal and then ...

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    \Thanks for your quick answer.
    I'll be happy to try another antibody, but since we don't have secondary anti-sheep Ab, I would prefer not to use the ab35341. Instead, I thought of 40630 or 53040, which are also polyclonal and raised against a long se...

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    Thank you for your reply.
    I can send one of the rabbit polyclonal antibodies ab40630 or ab53040, but their immunogens have lower homology to the rat protein so they may show a weaker cross-reactivity. I certainly understand that a rabbit polyclonal w...

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    The customer agrees to proceed with the abreview promotion with ab111097 for WB with rat samples. Please issue the discount code.

    Thank you for your reply.

    Would you please provide the end user information so that we can confirm the Abreview submission? Thank you in advance.

    I am very pleased to hear you would like to accept our offer and test ab111097 in WB wi...

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    Dear Tech Support Team, Our customer is willing to test ab53040 in WB with rat samples. Are you interested in this type of the information for the Abreview promotion (The customer buys certain antibodies and upload Abreview on Abcam's website and then we ...

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    Thank you for contacting Abcam regarding ab53040.

    I have aligned the immunogen sequence with the mouse sequence and unfortunately, there is only 60% homology between the two. We would not predict that this antibody will cross react with the rat...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"