Anti-Parathyroid Hormone antibody (ab53040)

Overview

  • Product nameAnti-Parathyroid Hormone antibody
    See all Parathyroid Hormone primary antibodies
  • Description
    Rabbit polyclonal to Parathyroid Hormone
  • SpecificityThis antibody detects endogenous levels of total parathyroid hormone
  • Tested applicationsSandwich ELISA, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from human Parathyroid Hormone corresponding to amino acid 71 to 100.

  • Positive control
    • A549 cell extract

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°C
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab53040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [A1/70] to Parathyroid Hormone (ab14493). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab14493 as Capture.
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 13 kDa).
ELISA 1/10000.

Target

  • FunctionPTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • Involvement in diseaseDefects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
  • Sequence similaritiesBelongs to the parathyroid hormone family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPTH antibody
    • Parathormone antibody
    • Parathyrin antibody
    • Parathyroid hormone 1 antibody
    • Parathyroid hormone antibody
    • PTH antibody
    • PTH1 antibody
    • PTH1 receptor antibody
    • PTH1R antibody
    • PTHR antibody
    • PTHR1 antibody
    • PTHY_HUMAN antibody
    see all

Anti-Parathyroid Hormone antibody images

Protocols

References for Anti-Parathyroid Hormone antibody (ab53040)

ab53040 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

The amino acid range of the immunogen for ab53040 is aa50-100 of human parathyroid hormone with accession number P01270 (http://www.uniprot.org/uniprot/P01270).

If you could provide the exact protein sequen...

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Thank you for your email.

We generally add the location of immunogen e.g. N-terminal, C-terminal or internal on datasheet however I do agree this wasn't the case for this ab53040. This antibody would pick up the whole PTH but not 1-34 aa pepti...

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Thank you for your email.

The immunogen of antibody ab53040 is a sequence from amino acid 71 to aa 100 which might be the reason this antibody is not detecting band of interest.

ab40630 is indeed tested for 100% reactivity with 1-34 p...

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Thank you very much for your reply and for the information.
I have added a vial of ab40630 to a shipment and forwarded the details of this replacement to your contact.
I will be back in touch regarding ab53040, and if there is anything else th...

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Thank you very much for your reply and for sending the information about the PTHrP expression in A549 cells.
I will follow-up with the lab about ab53040, and I am happy to send a vial of ab40630. I will need to send this vial first to Zotal and th...

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Thank you for your reply.
I can send one of the rabbit polyclonal antibodies ab40630 or ab53040, but their immunogens have lower homology to the rat protein so they may show a weaker cross-reactivity. I certainly understand that a rabbit polyclona...

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Thank you for your reply.

Would you please provide the end user information so that we can confirm the Abreview submission? Thank you in advance.

I am very pleased to hear you would like to accept our offer and test ab111097 in WB...

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Thank you for contacting Abcam regarding ab53040.

I have aligned the immunogen sequence with the mouse sequence and unfortunately, there is only 60% homology between the two. We would not predict that this antibody will cross react with the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"