Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Parkin / PARK
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Ab77924 is not showing a band in Western blot and ab15954 gives a band in knockdown samples. |
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Thank you for your call today and for letting us know about the trouble with these antibodies. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-Parkin antibody [PRK8] (ab77924) at 1/2000 dilution + HEK293 cell lysate in RIPA buffer
Secondary
Goat anti-Mouse HRP conjugated at 1/5000 dilution
developed using the ECL technique
Predicted band size : 52 kDa
Observed band size : 52 kDa
ICC/IF image of PC12 cell line culture stained with Ab77924. The cells on cover slip were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3x triton1000 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77924, 1 µg/ml) overnight at +4ºC. The secondary antibody was Alexa Fluor®488 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
ab77924 staining Parkin in murine myocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed with 4% paraformaldehyde, permeabilized with 1% Triton X-100, blocked with 10% serum for 1 hour at room temperature and then incubated with ab79924 at a 1/200 dilution for 1 hour. The secondary used was and Alexa-Fluor 488 conjugated goat anti-rabbit, used at a 1/500 dilution. Counterstained with DAPI.Upper panel cells are untreated.Lower panel showed that parkin translocated from cyto to mito in mycoytes when treated with FCCP.
Image courtesy of an anonymous Abreview.
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