Parkin antibody (ab15954)

Product Name 

Parkin antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Parkin

Immunogen 

Synthetic peptide: RILGEEQYTRYQQYGAEEC conjugated to KLH, corresponding to amino acids 304-322 of Mouse Parkin.
BLAST 'RILGEEQYTRYQQYGAEEC' with or

Reacts with 
(species key)

Hu, Ms, Rat, Cow, Mk

Tested applications 
(see key)

ICC/IF, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: 1/1000.
IF: 1/1000.
IHC-Fr: Use at an assay dependent dilution.
IP: 1/200.
WB: 1/1000 - 1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 51.6 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Use HEK293, SH-SY5Y or COS7 cell lysate.

Cellular localization 

Cytoplasmic

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Parkin / PARK

Relevance 

Parkinson's Disease, the second most common neurodegenerative disease after Alzheimer's Disease, is characterized by the loss of dopaminergic neurons and the presence of Lewy bodies (comprised of alpha synuclein and parkin inclusions). Autosomal Recessive Juvenile Parkinsonism (AR-JP) is a recently described form of Parkinson's Disease that has been linked to a gene that codes for parkin. Parkin, a 52 kDa protein, has a suggested role in the ubiquitin/proteasome pathway for protein degradation. The amino terminus bears sequence homology to ubiquitin while functionally it acts as a RING type ubiquitin protein ligase (E3) that coordinates the transfer of ubiquitin to substrate proteins, thus targeting them for degradation by the proteasome.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified by affinity chromatography using the immunising peptide immobilized on SulfoLink gel matrix (Pierce).

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Parkin antibody images, references, products related to ab15954 and other tools.

Parkin antibody images:

References for Parkin antibody (ab15954)

  This product has been used in: (two most recent references)

Vives-Bauza C et al. PINK1-dependent recruitment of Parkin to mitochondria in mitophagy. Proc Natl Acad Sci U S A 107:378-83 (2010). WB, ICC/IF; Human. PubMed: 19966284

Geisler S et al. PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1. Nat Cell Biol 12:119-31 (2010). ICC/IF, IP; Human. PubMed: 20098416

See all 13 publication references for this product.


If you publish research using ab15954 please let us know so that we can cite the reference on this datasheet.

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