Anti-Parkin antibody (ab55426)

Overview

• Product nameAnti-Parkin antibodySee all Parkin primary antibodies ...
• Description
  Rabbit polyclonal to Parkin
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic peptide (Human)

• Positive controlHuman brain tissue, extracts from Jurkat cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitophagy fission and fusion

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Parkinson's disease
  • Parkin / PARK

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin E2s and E3s
  • RING Finger E3 Ligase

Applications

Target

• FunctionFunctions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP, SEPT5, ZNF746 and AIMP2. Mediates monoubiquitination as well as 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation. Mediates 'Lys-63'-linked polyubiquitination of SNCAIP, possibly playing a role in Lewy-body formation. Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy. Promotes the autophagic degradation of dysfunctional depolarized mitochondria. Mediates 'Lys-48'-linked polyubiquitination of ZNF746, followed by degradation of ZNF746 by the proteasome; possibly playing a role in role in regulation of neuron death. Limits the production of reactive oxygen species (ROS). Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of PARK2. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Regulates cyclin-E during neuronal apoptosis. May represent a tumor suppressor gene.
• Tissue specificityHighly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle. Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons from kainate-mediated apoptosis. Found in serum (at protein level).
• PathwayProtein modification; protein ubiquitination.
• Involvement in diseaseDefects in PARK2 are a cause of Parkinson disease (PARK) [MIM:168600]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. Additional features are characteristic postural abnormalities, dysautonomia, dystonic cramps, and dementia. The pathology of Parkinson disease involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. The disease is progressive and usually manifests after the age of 50 years, although early-onset cases (before 50 years) are known. The majority of the cases are sporadic suggesting a multifactorial etiology based on environmental and genetic factors. However, some patients present with a positive family history for the disease. Familial forms of the disease usually begin at earlier ages and are associated with atypical clinical features.
  Defects in PARK2 are the cause of Parkinson disease type 2 (PARK2) [MIM:600116]; also known as early-onset parkinsonism with diurnal fluctuation (EPDF) or autosomal recessive juvenile Parkinson disease (PDJ). A neurodegenerative disorder characterized by bradykinesia, rigidity, postural instability, tremor, and onset usually befor 40. It differs from classic Parkinson disease by early DOPA-induced dyskinesia, diurnal fluctuation of the symptoms, sleep benefit, dystonia and hyper-reflexia. Dementia is absent. Pathologically, patients show loss of dopaminergic neurons in the substantia nigra, similar to that seen in Parkinson disease; however, Lewy bodies (intraneuronal accumulations of aggregated proteins) are absent.
  Note=Defects in PARK2 may be involved in the development and/or progression of ovarian cancer.
• Sequence similaritiesBelongs to the RBR family. Parkin subfamily.
  Contains 1 IBR-type zinc finger.
  Contains 2 RING-type zinc fingers.
  Contains 1 ubiquitin-like domain.
• DomainThe ubiquitin-like domain binds the PSMD4 subunit of 26S proteasomes.
• Post-translational
  modificationsAuto-ubiquitinates in an E2-dependent manner leading to its own degradation. Also polyubiquitinated by RNF41 for proteasomal degradation.
  S-nitrosylated. The inhibition of PARK2 ubiquitin E3 ligase activity by S-nitrosylation could contribute to the degenerative process in PD by impairing the ubiquitination of PARK2 substrates.
• Cellular localizationCytoplasm > cytosol. Nucleus. Endoplasmic reticulum. Mitochondrion. Mainly localizes in the cytosol. Co-localizes with SYT11 in neutrites. Co-localizes with SNCAIP in brainstem Lewy bodies. Relocates to dysfunctional mitochondria that have lost the mitochondial membrane potential; recruitement to mitochondria is PINK1-dependent.

Target information above from: UniProt accession O60260 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5071 Human
  • Entrez Gene: 50873 Mouse
  • Entrez Gene: 56816 Rat
  • Omim: 602544 Human
  • SwissProt: O60260 Human
  • SwissProt: Q9WVS6 Mouse
  • SwissProt: Q9JK66 Rat
  • Unigene: 132954 Human

  • Unigene: 311110 Mouse
  • Unigene: 207194 Rat

  see all

• Alternative names
  AR JP antibodyE3 ubiquitin ligase antibodyE3 ubiquitin protein ligase parkin antibody

  E3 ubiquitin-protein ligase parkin antibodyFRA6E antibodyLPRS 2 antibodyLPRS2 antibodyPARK 2 antibodyPARK2 antibodyParkin 2 antibodyParkinson disease (autosomal recessive juvenile) 2 antibodyParkinson disease protein 2 antibodyParkinson juvenile disease protein 2 antibodyParkinson protein 2 E3 ubiquitin protein ligase antibodyPDJ antibodyPRKN 2 antibodyPRKN antibodyPRKN2 antibodyPRKN2_HUMAN antibodyUbiquitin E3 ligase PRKN antibody

  see all

Anti-Parkin antibody images

• 

  Western blot - Parkin antibody (ab55426)
  All lanes : Anti-Parkin antibody (ab55426) at 1/500 dilution
  
  Lane 1 : extracts from Jurkat cells
  Lane 2 : extracts from Jurkat cells with immunizing peptide
  
  
  Predicted band size : 52 kDa
  Observed band size : 52 kDa
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - Parkin antibody (ab55426)
  Ab55426 at 1/50 dilution staining human brain tissue without and with immunizing peptide; paraffin embedded.