Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Proteins and Peptides
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Pax2 antibody - ChIP Grade
See all Pax2 products (6) ...
Description
Rabbit polyclonal to Pax2 - ChIP Grade
Tested applications
IHC-P, ICC/IF, WB, ChIPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Zebrafish
Immunogen
A region within synthetic peptide: DPVGSYSING ILGIPRSNGE KRKRDEDVSE GSVPNGDSQS GVDSLRKHLR, corresponding to internal sequence amino acids 180-229 of Human Pax2
DPVGSYSINGILGIPRSNGEKRKRDEDVSEGSVPNGDSQS GVDSLRKHLR
Positive control
IHC-P: Human kidney. ICC: OVCA429 cells (see review) WB: OVCAR-5, OVCAR-3, SKOV3, CaoV3 cell lysates (See review)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> PAX
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF: 1/250. Fix with 3% paraformaldehyde/2% sucrose (see review by R Drapkin).
WB: Use a concentration of 1 - 2.5 µg/ml. Detects a band of approximately 34 , 41 kDa (predicted molecular weight: 44 , 42 kDa).Dilute antibody in 5% milk in PBS buffer. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ChIP: Use at an assay dependent dilution.
Target
Relevance
PAX2 is a transcription factor critically required during the development of the nervous and excretory systems, including the midbrain, hindbrain, spinal cord, eye, ear and urogenital tract. Like other products of the PAX gene family, PAX2 encodes a conserved 128 amino acid paired box DNA-binding domain in the N-terminal portion of the molecule.
Cellular localization
Nuclear
Alternative names
- Paired box 2 antibody
- Paired box gene 2 antibody
- paired box homeotic gene 2 antibody
see all
Database links
- Entrez Gene: 5076 Human
- Entrez Gene: 18504 Mouse
- Entrez Gene: 293992 Rat
- Omim: 167409 Human
- SwissProt: Q02962 Human
- SwissProt: P32114 Mouse
- Unigene: 155644 Human
Anti-Pax2 antibody - ChIP Grade images:
Western blot - Pax2 antibody (ab23799)
Anti-Pax2 antibody - ChIP Grade (ab23799) at 2.5 µg/ml + Fetal kidney lysate
Predicted band size : 44 , 42 kDa
Observed band size : 34,41 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - Pax2 antibody (ab23799)
ab23799, at an antibody concentration of 4.0 - 8.0 µg/ml, staining Human PAX2 in paraffin embeded kidney tissue. The arrows indicate the positively labelled cells, epithelial cells of the renal tube.
Immunocytochemistry/ Immunofluorescence - Pax2 antibody (ab23799)
The human ovarian carcinoma cell line OVCA429 was used with ab23799 in ICC/IF. The cells were fixed in paraformaldehyde. Blocking was in 5% serum for 1 hour. The primary antibody was incubated at a dilution of 1/250 for one hour. Immunofluorescence (ICC/IF) only worked with 3% paraformaldehyde/2% sucrose fixation. When 70% methanol/30% acetone fixation was tried non-specific cytoplasmic staining was seen. In the left hand panel staining with DAPI is shown, and in the right staining with the ab23799 antibody.
This image was taken from an Abreview submitted on January 5, 2006 from Ron Drapkin. We do not have any further information relating to this image.
References for Anti-Pax2 antibody - ChIP Grade (ab23799)
This product has been referenced in:
- Lee SB et al. PAX2 Regulates ADAM10 Expression and Mediates Anchorage-Independent Cell Growth of Melanoma Cells. PLoS One 6:e22312 (2011). WB; Human.Read more (PubMed: 21876729) »
- Yu AL et al. Reactivation of optic nerve head astrocytes by TGF-beta2 and H2O2 is accompanied by increased Hsp32 and Hsp47 expression. Invest Ophthalmol Vis Sci 50:1707-17 (2009). ICC/IF; Human.Read more (PubMed: 18952926) »
See all 4 publications for this product
Publishing research using ab23799? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"