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- Proteins and Peptides
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Product name
Anti-Pax2 antibody
See all Pax2 products (6) ...
Description
Rabbit polyclonal to Pax2
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Chicken, Human, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse Pax2.
(Peptide available as ab391 08.)
Positive control
This antibody gives a positive signal in the following lysates: Mouse Fetal lysate - 14 days old tissue lysate Brain (Mouse) Whole Cell Lysate - normal tissue, 0 days old Mouse Embryonic Fibroblast 1 Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> PAX
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/250. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ICC/IF: Use a concentration of 1 µg/ml.
Target
Relevance
PAX2 is a transcription factor critically required during the development of the nervous and excretory systems, including the midbrain, hindbrain, spinal cord, eye, ear and urogenital tract. Like other products of the PAX gene family, PAX2 encodes a conserved 128 amino acid paired box DNA-binding domain in the N-terminal portion of the molecule.
Cellular localization
Nuclear
Alternative names
- Paired box 2 antibody
- Paired box gene 2 antibody
- paired box homeotic gene 2 antibody
see all
Database links
- Entrez Gene: 5076 Human
- Entrez Gene: 18504 Mouse
- Omim: 167409 Human
- SwissProt: Q02962 Human
- SwissProt: P32114 Mouse
- Unigene: 155644 Human
Anti-Pax2 antibody images:
Western blot - Pax2 antibody (ab38738)
All lanes : Anti-Pax2 antibody (ab38738) at 1/250 dilution Pax2 is not expressed in skeletal muscle.
Lane 1 : Mouse Fetal lysate - 14 Days old Tissue Lysate
Lane 2 :
Lane 3 : Mouse Embryonic Fibroblast 1 Whole Cell Lysate
Lane 4 :
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 44 kDa
Observed band size : 44 kDa
Additional bands at : 25 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Pax2 antibody (ab38738)
ICC/IF image of ab38738 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38738, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Pax2 antibody (ab38738)
This product has been referenced in:
- Treviño LS et al. Gene expression profiling reveals differentially expressed genes in ovarian cancer of the hen: support for oviductal origin? Horm Cancer 1:177-86 (2010). IHC-P; Chicken.Read more (PubMed: 21761365) »
- Hurtado A et al. Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen. Nature 456:663-6 (2008). WB, IHC-P; Human.Read more (PubMed: 19005469) »
See all 2 publications for this product
Publishing research using ab38738? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"