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Product name
Anti-Paxillin antibody
See all Paxillin products (24) ...
Description
Rabbit polyclonal to Paxillin
Specificity
Ab39537 detects endogenous levels of total Paxillin protein.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
This antibody was produced against synthesized non-phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 118.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Predicted molecular weight: 65 kDa.
ELISA
ELISA: 1/20000.ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
Target
Function
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similarities
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
Post-translational
modifications
Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Cellular localization
Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
Target information above from: UniProt accessionP49023
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
see all
Database links
- Entrez Gene: 5829 Human
- Entrez Gene: 19303 Mouse
- Entrez Gene: 360820 Rat
- Omim: 602505 Human
- SwissProt: P49023 Human
- SwissProt: Q8VI36 Mouse
- SwissProt: Q66H76 Rat
- Unigene: 446336 Human
see all
Anti-Paxillin antibody images:
Western blot - Paxillin antibody (ab39537)
Lanes 1 - 2 : Anti-Paxillin antibody (ab39537)
Lanes 3 - 4 : Anti-Paxillin (phospho Y118) antibody (ab38466)
Lane 1 : HeLa Cells
Lane 2 : HeLa Cells
Lane 3 : HeLa Cells
Lane 4 : HeLa Cells
Predicted band size : 65 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - Paxillin antibody (ab39537)
Ab39537 staining human Paxillin in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
References for Anti-Paxillin antibody (ab39537)
ab39537 has not yet been referenced specifically in any publications.
Publishing research using ab39537? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"