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Product name
Anti-Paxillin (phospho Y118) antibody
See all Paxillin products (24) ...
Description
Rabbit polyclonal to Paxillin (phospho Y118)
Specificity
This antibody is specific for Paxillin only when phosphorylated at tyrosine 118.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from Human Paxillin around the phosphorylation site of tyrosine 118 (HVYpSF).
Positive control
Hela cell extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
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Western blot - Paxillin (phospho Y118) antibody (ab38466)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: 1/10,000.
WB: 1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similarities
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
Post-translational
modifications
Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Cellular localization
Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
Target information above from: UniProt accessionP49023
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ16691 antibody
- FLJ23042 antibody
- FLJ23042 antibody
see all
Database links
- Entrez Gene: 5829 Human
- Entrez Gene: 19303 Mouse
- Entrez Gene: 360820 Rat
- Omim: 602505 Human
- SwissProt: P49023 Human
- SwissProt: Q8VI36 Mouse
- SwissProt: Q66H76 Rat
- Unigene: 446336 Human
see all
Anti-Paxillin (phospho Y118) antibody images:
Western blot - Paxillin (phospho Y118) antibody (ab38466)
All lanes : Anti-Paxillin (phospho Y118) antibody (ab38466) at 1/500 dilution
Lane 1 : Hela cell extract + EGF (Cells were treated with 200ng/ml EGF for 20 minutes)
Lane 2 : Hela cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size : 64 kDa
Lanes can be loaded with 5-30µg of total protein.
References for Anti-Paxillin (phospho Y118) antibody (ab38466)
ab38466 has not yet been referenced specifically in any publications.
Publishing research using ab38466? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"